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Cloning and expression of a codon-optimized gene encoding the influenza A virus nucleocapsid protein in Lactobacillus casei

AuthorsSuebwongsa, Namfon; Panya, Marutpong; Namwat, Wises; Sookprasert, Saovaluk; Redruello, Begoña ; Mayo Pérez, Baltasar ; Álvarez González, Miguel Ángel ; Lulitanond, Viraphong
KeywordsInfluenza A virus
Lactic acid bacteria
Viral nucleocapsid proteins
Heterologous expression
Codon usage
Lactobacillus casei
Issue Date2013
PublisherHemeroteca Científica Catalana
CitationInternational Microbiology 16: 93-101 (2013)
AbstractLactic acid bacteria (LAB) species are envisioned as promising vehicles for the mucosal delivery of therapeutic and prophylactic molecules, including the development of oral vaccines. In this study, we report on the expression of a synthetic nucleocapsid (NP) gene of influenza A virus in Lactobacillus casei. The NP gene was re-designed based on the tRNA pool and the codon usage preference of L. casei BL23. The codon-optimized NP gene was then cloned and expressed in L. casei RCEID02 under the control of a constitutive promoter, that of the lactate dehydrogenase (ldh) gene. The synthetic NP gene was further expressed in L. casei EM116 under the control of an inducible promoter, that of the structural gene of nisin (nisA) from Lactococcus lactis. Based on Western blot analysis, the specific protein band of NP, with a molecular mass of 56.0 kDa, was clearly detected in both expression systems. Thus, our study demonstrates the success of expressing a codon-optimized influenza A viral gene in L. casei. The suitability of the recombinant LAB strains for immunization purposes is currently under evaluation.
Publisher version (URL)http://dx.doi.org/10.2436/20.1501.01.184
Identifiersdoi: 10.2436/20.1501.01.184
issn: 1139-6709
e-issn: 1618-1905
Appears in Collections:(IPLA) Artículos
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