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dc.contributor.authorBergua, Maria-
dc.contributor.authorZwart, Mark P.-
dc.contributor.authorEl-Mohtar, Choaa-
dc.contributor.authorShilts, Turksen-
dc.contributor.authorElena, Santiago F.-
dc.contributor.authorFolimonova, Svetlana Y.-
dc.identifier.citationJournal of Virology (88): 11327-11338 (2014)es_ES
dc.description.abstractSuperinfection exclusion (SIE), the ability of an established virus infection to interfere with a secondary infection by the same or a closely related virus, has been described for different viruses, including important pathogens of humans, animals, and plants. Citrus tristeza virus (CTV), a positive-sense RNA virus, represents a valuable model system for studying SIE due to the existence of several phylogenetically distinct strains. Furthermore, CTV allows SIE to be examined at the whole-organism level. Previously, we demonstrated that SIE by CTV is a virus-controlled function that requires the viral protein p33. In this study, we show that p33 mediates SIE at the whole-organism level, while it is not required for exclusion at the cellular level. Primary infection of a host with a fluorescent protein-tagged CTV variant lacking p33 did not interfere with the establishment of a secondary infection by the same virus labeled with a different fluorescent protein. However, cellular coinfection by both viruses was rare. The obtained observations, along with estimates of the cellular multiplicity of infection (MOI) and MOI model selection, suggested that low levels of cellular coinfection appear to be best explained by exclusion at the cellular level. Based on these results, we propose that SIE by CTV is operated at two levels-the cellular and the whole-organism levels-by two distinct mechanisms that could function independently. This novel aspect of viral SIE highlights the intriguing complexity of this phenomenon, further understanding of which may open up new avenues to manage virus diseases.es_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.titleA Viral Protein Mediates Superinfection Exclusion at the Whole-Organism Level but Is Not Required for Exclusion at the Cellular Leveles_ES
dc.description.peerreviewedPeer reviewedes_ES
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