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Título

Protein carbonylation associated to high-fat, high-sucrose diet and its metabolic effects

AutorMéndez, Lucía CSIC ORCID; Pazos Palmeiro, Manuel CSIC ORCID; Molinar-Toribio, Eunice; Sánchez-Martos, Vanessa; Gallardo, José Manuel CSIC; Nogués, M. R.; Torres, Josep Lluís CSIC ORCID; Medina, Isabel CSIC ORCID
Palabras claveHigh-fat, high-sucrose diet
Obesity
Insulin resistance
NAFLD
Protein carbonylation
Sprague–Dawley rat
Fecha de publicación2014
EditorElsevier
CitaciónJournal of Nutritional Biochemistry 25(12): 1243-1253 (2014)
ResumenThe present research draws a map of the characteristic carbonylation of proteins in rats fed high-caloric diets with the aim of providing a new insight of the pathogenesis of metabolic diseases derived from the high consumption of fat and refined carbohydrates. Protein carbonylation was analyzed in plasma, liver and skeletal muscle of Sprague–Dawley rats fed a high-fat, high-sucrose (HFHS) diet by a proteomics approach based on carbonyl-specific fluorescence-labeling, gel electrophoresis and mass spectrometry. Oxidized proteins along with specific sites of oxidative damage were identified and discussed to illustrate the consequences of protein oxidation. The results indicated that long-term HFHS consumption increased protein oxidation in plasma and liver; meanwhile, protein carbonyls from skeletal muscle did not change. The increment of carbonylation by HFHS diet was singularly selective on specific target proteins: albumin from plasma and liver, and hepatic proteins such as mitochondrial carbamoyl-phosphate synthase (ammonia), mitochondrial aldehyde dehydrogenase, argininosuccinate synthetase, regucalcin, mitochondrial adenosine triphosphate synthase subunit beta, actin cytoplasmic 1 and mitochondrial glutamate dehydrogenase 1. The possible consequences that these specific protein carbonylations have on the excessive weight gain, insulin resistance and nonalcoholic fatty liver disease resulting from HFHS diet consumption are discussed.
Descripción11 páginas, 4 tablas, 7 figuras
Versión del editorhttp://dx.doi.org/10.1016/j.jnutbio.2014.06.014
URIhttp://hdl.handle.net/10261/108524
DOI10.1016/j.jnutbio.2014.06.014
ISSN0955-2863
E-ISSN1873-4847
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