Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/107814
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Signaling role for lysophosphatidylcholine acyltransferase 3 in receptor-regulated arachidonic acid reacylation reactions in human monocytes

AutorPérez-Chacón, Gema CSIC ORCID; Astudillo, Alma M. CSIC ORCID; Ruipérez, Violeta CSIC ORCID CVN; Balboa, María A. CSIC ORCID; Balsinde, Jesús CSIC ORCID
Fecha de publicación2010
EditorAmerican Association of Immunologists
CitaciónJournal of Immunology 184(2): 1071-1078 (2010)
ResumenCellular availability of free arachidonic acid (AA) is an important step in the production of pro- and anti-inflammatory eicosanoids. Control of free AA levels in cells is carried out by the action of phospholipase A2s and lysophospholipid acyltransferases, which are responsible for the reactions of deacylation and incorporation of AA from and into the sn-2 position of phospholipids, respectively. In this work, we have examined the pathways for AA incorporation into phospholipids in human monocytes stimulated by zymosan. Our data show that stimulated cells exhibit an enhanced incorporation of AA into phospholipids that is not secondary to an increased availability of lysophospholipid acceptors due to phospholipase A2 activation but rather reflects the receptor-regulated nature of the AA reacylation pathway. In vitro activity measurements indicate that the receptor-sensitive step of the AA reacylation pathway is the acyltransferase using lysophosphatidylcholine (lysoPC) as acceptor, and inhibition of the enzyme lysoPC acyltransferase 3 by specific small interfering RNA results in inhibition of the stimulated incorporation of AA into phospholipids. Collectively, these results define lysoPC acyltransferase 3 as a novel-signal-regulated enzyme that is centrally implicated in limiting free AA levels in activated cells. Copyright © 2010 by The American Association of Immunologists, Inc.
URIhttp://hdl.handle.net/10261/107814
DOI10.4049/jimmunol.0902257
Identificadoresdoi: 10.4049/jimmunol.0902257
issn: 0022-1767
e-issn: 1550-6606
Aparece en las colecciones: (IBGM) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
Signaling Role for LPCAT3_Pérez_Preprint_2010.pdf291,87 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

52
checked on 14-mar-2024

WEB OF SCIENCETM
Citations

54
checked on 29-feb-2024

Page view(s)

305
checked on 18-mar-2024

Download(s)

6
checked on 18-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.