DSpace

Digital.CSIC > Ciencia y Tecnologías Químicas > Instituto de Diagnóstico Ambiental y Estudios del Agua (IDAEA) > (IDAEA) Artículos >

Share

EndNote

Impact

Links

Closed Access item Alternative calibration approaches for LC-MS quantitative determination of coeluted compounds in complex environmental mixtures using multivariate curve resolution

Authors:Peré-Trepat, Emma
Lacorte Bruguera, Silvia
Tauler Ferré, Romà
Keywords:Multivariate curve resolution, HPLC-MS, Internal standard, Standard addition, Calibration approaches, Coelution, Environmental samples
Issue Date:13-Apr-2007
Publisher:Elsevier
Citation:Analytica Chimica Acta 595(1-2): 228-237 (2007)
Abstract:Different calibration approaches including external calibration, standard addition and internal standard are evaluated for quantification of coeluted compounds in liquid chromatography with MS spectrometry detection in scan mode and using multivariate curve resolution. These different calibration approaches are proposed to cope with sensitivity changes and matrix effects encountered in the analysis of complex natural environmental samples. By using them, multivariate curve resolution analysis of MS data in scan mode gave similar quantitative results to those obtained by LC–MS in selected ion monitoring (SIM) mode (in both cases errors were below 16% for internal standard combined with standard addition strategy), and it provided at the same time a means of analyte confirmation via their resolved pure MS spectra, and a means to gather a larger amount of information about the whole chromatographic process and to facilitate the simultaneous determination of multiple analytes in the same chromatographic run using the same experimental and instrumental conditions.
Description:10 pages, 2 tables, 4 figures.-- PMID: 17606005 [PubMed].-- Printed version published Jul 9, 2007.-- Issue title: Papers presented at the 10th International Conference on Chemometrics in Analytical Chemistry - CAC 2006 (Campinas, Sao Paulo, Sep 10-14, 2006).
Publisher version (URL):http://dx.doi.org/10.1016/j.aca.2007.04.011
URI:http://hdl.handle.net/10261/10652
ISSN:0003-2670
???metadata.dc.identifier.doi???:10.1016/j.aca.2007.04.011
Appears in Collections:(IDAEA) Artículos

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.