English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/10636
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
DC FieldValueLanguage
dc.contributor.authorTestillano, P.S.-
dc.contributor.authorGonzález-Melendi, Pablo-
dc.contributor.authorCoronado, María José-
dc.contributor.authorSeguí-Simarro, José María-
dc.contributor.authorMoreno, Miguel A.-
dc.contributor.authorRisueño, María Carmen-
dc.identifier.citationCytogenetic and Genome Research 109(1-3):166-174(2005)en_US
dc.description9 pages.-- PMID: 15753573 [PubMed].-- Issue title: Plant Cytogenetics, eds. Maria J. Puertas, Madrid; Tomäs Naranjo, Madrid.en_US
dc.description.abstractThe immature pollen grain, the microspore, under stress conditions can switch its developmental program towards proliferation and embryogenesis. The comparison between the gametophytic and sporophytic pathways followed by the microspore permitted us to analyse the nuclear changes in plant differentiating cells when switched to proliferation. The nucleus is highly dynamic, the architecture of its well organised functional domains - condensed chromatin, interchromatin region, nuclear bodies and nucleolus - changing in response to DNA replication, RNA transcription, processing and transport. In the present work, the rearrangements of the nuclear domains during the switch to proliferation have been determined by in situ molecular identification methods for the subcellular localization of chromatin at different functional states, rDNA, elements of the nuclear machinery (PCNA, splicing factors), signalling and stress proteins. The study of the changes in the nuclear domains was determined by a correlative approach at confocal and electron microscopy levels. The results showed that the switch of the developmental program and the activation of the proliferative activity affected the functional organization of the nuclear domains, which accordingly changed their architecture and functional state. A redistribution of components, among them various signalling molecules which targeted structures within the interchromatin region upon translocation from the cytoplasm, was also observed.en_US
dc.description.sponsorshipSupported by Projects of Spanish MCyT (BOS2002-03572), Comunidad de Madrid (07G/0026/2003) and Spanish-Czech Joint project (CSIC-CAS 2003CZ0019). P.G.-M. is a Research Associate of the C.S.I.C. funded by the program "Ramón y Cajal" of the Spanish Ministry of Science and Technology.en_US
dc.format.extent173568 bytes-
dc.publisherS. Karger AGen_US
dc.titleDifferentiating plant cells switched to proliferation remodel the functional organization of nuclear domainsen_US
dc.description.peerreviewedPeer revieweden_US
Appears in Collections:(CIB) Artículos
Files in This Item:
There are no files associated with this item.
Show simple item record

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.