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Title

Tracking gene and protein expression during microspore embryogenesis by Confocal Laser Scanning Microscopy

AuthorsTestillano, P.S. ; Risueño, María Carmen
KeywordsMicrospore embryogenesis
Confocal laser scanning microscopy
3D analysis
Bioimaging
Gene and protein expression
Tracking
FISH
Immunofluorescence
Stress
Signalling
Issue Date18-Dec-2008
PublisherSpringer
CitationAdvances in Haploid Production in Higher Plants, Alisher Touraev, Brain P. Forster and S. Mohan Jain (eds.), pp. 339-347
AbstractConfocal Laser Scanning Microscopy (CLSM) technology and bioimaging are powerful tools for three-dimensional (3D) and colocalization molecular analysis of the microspore embryogenesis. Strategies with fluorescent-labelled probes for in situ hybridization and immunofluorescence have provided unique images of the spatial and temporal pattern of the expression of genes and proteins, and of the sub-cellular rearrangements that accompany the microspore embryogenesis. Various signalling and stress proteins were differentially expressed in reprogrammed microspores and young embryos, and specific endosperm and embryo genes were expressed at different stages, supporting the existence of an endosperm-like domain, in cereals. Specific features such as changes in cell wall components and pectin esterification, presence of callose in special walls, and different behaviour of Cajal nuclear bodies were found in embryogenic microspores and young embryos, constituting early embryogenic markers. The 3D analysis of the nuclear dynamics at early stages of microspore embryogenesis has proved that the nuclear fusion was the mechanism of the spontaneous diploidization.
Description9 pages, 2 figures.
Publisher version (URL)http://dx.doi.org/10.1007/978-1-4020-8854-4_28
URIhttp://hdl.handle.net/10261/10618
DOI10.1007/978-1-4020-8854-4_28
ISBN978-1-4020-8853-7 (Print)
978-1-4020-8854-4 (Online)
Appears in Collections:(CIB) Libros y partes de libros
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