English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/10605
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Early markers of in vitro microspore reprogramming to embryogenesis in olive (Olea europaea L.)

AuthorsSolís, María Teresa ; Pintos, Beatriz; Prado, María Jesús; Bueno, María Ángeles; Raska, Ivan; Risueño, María Carmen ; Testillano, P.S.
Pollen embryogenesis
Cell wall
Cellular markers
Microspore culture
Issue Date30-Mar-2008
CitationPlant Science 174(6):597-605(2008)
AbstractMicrospore embryogenesis to form haploid and double-haploid embryos and regenerated plants is an efficient method of producing homozygous lines for crop breeding. In trees, the process is of special interest since classical methods are impractical in many cases, as in Olea europaea L. Recently, a convenient method has been developed for microspore embryogenesis induction by stress in olive isolated microspores in vitro cultures. In the present work, the switch of the microspore developmental pathway and the formation of microspore-derived multicellular proembryos have been achieved and a cytochemical and immunocytochemical analysis was performed in the early stages. The young microspore proembryos displayed defined features different to both, the in vivo gametophytic, and the in vitro non-responsive microspores. Reprogrammed microspores showed an absence of starch, the occurrence of a first symmetrical division and cytokinesis, the presence of an abundant ribosomal population, and changes in cellulosic and pectic cell wall components which constituted early markers of the embryogenic microspore process. They provided new insights on the molecular and cellular events associated with the microspore reprogramming of woody plants, and specifically in olive, providing interesting knowledge which could guide future selection and regeneration strategies in this fruit tree of high economic interest.
Description9 pages, 6 figures.-- Printed version published Jun 2008.
Publisher version (URL)http://dx.doi.org/10.1016/j.plantsci.2008.03.014
Appears in Collections:(CIB) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.