Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/10503
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Mutagenesis of prochlorothrix plastocyanin reveals additional features in photosystem I interactions

AutorHervás, Manuel CSIC ORCID; Myshkin, Eugene; Vintonenko, Nadejda; Rosa, Miguel A. de la CSIC ORCID; Bullerjahn, George S.; Navarro, José A. CSIC ORCID
Fecha de publicación7-mar-2003
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónJournal of Biological Chemistry 278 (10): 8179-8183 (2003)
ResumenThree surface residues of plastocyanin from Prochlorothrix hollandica have been modified by site-directed mutagenesis. Changes have been made in methionine 33, located in the hydrophobic patch of the copper protein, and in arginine 86 and proline 53, both located in the eastern hydrophilic area. The reactivity toward photosystem I of single mutants M33N, P53A, P53E, R86Q, R86E, and the double mutant M33N/P14L has been studied by laser flash absorption spectroscopy. All the mutations yield increased reactivity of plastocyanin toward photosystem I as compared with wild type plastocyanin, thus indicating that in Prochlorothrix electron donation to photosystem I is not optimized. The most drastic increases in the intracomplex electron transfer rate are obtained with mutants in methionine 33, whereas replacing arginine 86 only modestly affects the plastocyanin-photosystem I equilibrium constant for complex formation. Mutations at position 53 also promote major changes in the association of plastocyanin with photosystem I, yielding a change from a mechanism involving complex formation to a simpler collisional interaction. Molecular dynamics calculations indicate that mutations at position 33 promote changes in the H-bond network around the copper center. The comparative kinetic analysis of the reactivity of Prochlorothrix plastocyanin mutants toward photosystem I from other cyanobacteria reveals that mutations M33N, P53A, and P53E result in enhanced general reactivity.
Descripción5 pages, 4 figures.-- PMID: 12509429 [PubMed].-- Onlise version published Dec 30, 2002.
Versión del editorhttp://dx.doi.org/10.1074/jbc.M211913200
URIhttp://hdl.handle.net/10261/10503
DOI10.1074/jbc.M211913200
ISSN0021-9258
Aparece en las colecciones: (IBVF) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

7
checked on 23-mar-2024

WEB OF SCIENCETM
Citations

7
checked on 27-feb-2024

Page view(s)

371
checked on 28-mar-2024

Download(s)

97
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.