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Title

Atomic force microscopy shows that vaccinia topoisomerase IB generates filaments on DNA in a cooperative fashion

AuthorsMoreno-Herrero, Fernando; Holtzer, Laurent; Koster, Daniel A.; Shuman, Stewart; Dekker, Cees; Dekker, Nynke H.
Issue Date19-Oct-2005
PublisherOxford University Press
CitationNucleic Acids Research 33(18): 5945-5953 (2005)
AbstractType IB DNA topoisomerases cleave and rejoin one strand of the DNA duplex, allowing for the removal of supercoils generated during replication and transcription. In addition, electron microscopy of cellular and viral TopIB–DNA complexes has suggested that the enzyme promotes long-range DNA–DNA crossovers and synapses. Here, we have used the atomic force microscope to visualize and quantify the interaction between vaccinia topoisomerase IB (vTopIB) and DNA. vTopIB was found to form filaments on nicked-circular DNA by intramolecular synapsis of two segments of a single DNA molecule. Measuring the filament length as a function of protein concentration showed that synapsis is a highly cooperative process. At high protein:DNA ratios, synapses between distinct DNA molecules were observed, which led to the formation of large vTopIB-induced DNA clusters. These clusters were observed in the presence of Mg2+, Ca2+ or Mn2+, suggesting that the formation of intermolecular vTopIB-mediated DNA synapsis is favored by screening of the DNA charge.
Description9 pages, 6 figures.-- PMID: 16237128 [PubMed].-- PMCID: PMC1258176.
Publisher version (URL)http://dx.doi.org/10.1093/nar/gki906
URIhttp://hdl.handle.net/10261/10463
DOI10.1093/nar/gki906
ISSN0305-1048
Appears in Collections:(CIN2) Artículos
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