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Enzymatic and molecular responses on the Manila clan, Ruditapes philippinarujm to gold nanoparticles . Evaluation of enzymatic and molecular endpoints

AuthorsBlasco, Julián ; Volland, Moritz; Hampel, Miriam ; García-Negrete, C. A. ; Lapresta-Fernández, A. ; Rojas, T. Cristina; Jiménez de Haro, María del Carmen ; Fernández-Camacho, A.
Issue DateSep-2011
Citation6th International Conference on the Environmental Effects of Nanoparticles and Nanomaterials (2011)
AbstractNanoparticles (NPs) are introduced into a growing number of commercial products, eventually leading to their release into the different environmental compartments. Up today, ecotoxicological risks in non target organisms associated with NPs are poorly documented. Thus in this study, the marine bivalve Ruditapes philippinarum was chosen as ecotoxicological model to evaluate the enzymatic and molecular biomarkers of effects of exposure to gold nanoparticles (AuNPs). The organisms were exposed in the laboratory to gold added to natural filtered seawater either in the form of citrale reduced AuNPs (approximately 6 and 30 ~g'L-1) in Ihe range of 20 - 30 nm or as soluble gold, H(AuCI4) (50 ~g'L-1) for 28 days. Samples (digestive gland, gills and mantle) were taken throughoul the exposure period in order to monitor. The development of Ihe expression of the chosen endpoints over time. Au concentrations were analysed in the three tissues. Biomarkers of oxidative stress (calalase, GPX, SOO, lipid peroxidalion) were quantified in digestive gland and gills. Metallothionein levels were evaluated by different approaches (HPLC and real time PCR). Additionally, different features involved in the inflammatory response, known to be induced by NPs in other organisms, were monitored by quantitative real time PCR Sections of gill and digestive gland tissue were observed under electron microscope (TEM/SEM) coupled to micro analysis (EOX). Our results show that exposure to AuNPs does not alter significantly the response of the selected biomarkers of oxidative stress in a clear way. However, dissolved gold showed lo alter the response of these endpoints.
DescriptionTrabajo presentado en la 6th International Conference on the Environmental Effects of Nanoparticles and Nanomaterials, celebrada en Londres del 19 al 21 de noviembre de 2011.
Appears in Collections:(ICMS) Comunicaciones congresos
(ICMAN) Comunicaciones congresos
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