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Título: | Optical immunosensor for fast and sensitive detection of DDT and related compounds in river water samples |
Autor: | Mauriz, Elba CSIC ORCID; Calle Martín, Ana CSIC; Manclús, J. J.; Montoya, Ángel; Hildebrandt, Alain CSIC; Barceló, Damià CSIC ORCID; Lechuga, Laura M. CSIC ORCID | Palabras clave: | Optical immunosensor Surface plasmon resonance (SPR) DDT Environmental analysis Cross-reactivity |
Fecha de publicación: | 15-feb-2007 | Editor: | Elsevier | Citación: | Biosensors & Bioelectronics 22(7): 1410-1418 (2007) | Resumen: | A surface plasmon resonance (SPR) based immunosensor has been developed for the monitoring of environmentally persistent pollutants like DDT, its metabolites and analogues in real water samples. A reusable immunosurface is provided via the covalent attachment of the analyte derivative to a self-assembled alkanethiol monolayer formed onto the SPR gold-thin layer. The regeneration of the sensor surface allowed the performance of 270 assay cycles within an analysis time of 20 min for each assay cycle. Immunoassays based on a binding inhibition format were performed by using two monoclonal antibodies (MAbs) with different selectivity. Low limits of detection (LODs), in the sub-nanogram per litre range, were attained for DDT-selective (15 ng L−1) and DDT group-selective immunoassays (31 ng L−1). Both assays were carried out in spiked river water samples without significant effect of the matrix. SPR measurements were validated using gas-chromatography–mass spectrometry. The comparison between methods was in good agreement showing an excellent correlation coefficient (r^2 = 0.995). The SPR analysis of DDT proved to be three times more sensitive than colorimetric ELISAs without the need of labelling and a much lower time of response. Our SPR biosensor portable platform (β-SPR) is already commercialised by the company SENSIA, S.L. (Spain). | Descripción: | 9 pages, 5 figures.-- PMID: 16870422 [PubMed].-- Available online Jul 25, 2006. | Versión del editor: | http://dx.doi.org/10.1016/j.bios.2006.06.016 | URI: | http://hdl.handle.net/10261/10347 | DOI: | 10.1016/j.bios.2006.06.016 | ISSN: | 0956-5663 |
Aparece en las colecciones: | (IMN-CNM) Artículos (IDAEA) Artículos |
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