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Closed Access item Optical immunosensor for fast and sensitive detection of DDT and related compounds in river water samples
Calle Martín, Ana
Manclús, J. J.
Lechuga, Laura M.
|Keywords:||Optical immunosensor, Surface plasmon resonance (SPR), DDT, Environmental analysis, Cross-reactivity|
|Citation:||Biosensors & Bioelectronics 22(7): 1410-1418 (2007)|
|Abstract:||A surface plasmon resonance (SPR) based immunosensor has been developed for the monitoring of environmentally persistent pollutants like DDT, its metabolites and analogues in real water samples. A reusable immunosurface is provided via the covalent attachment of the analyte derivative to a self-assembled alkanethiol monolayer formed onto the SPR gold-thin layer. The regeneration of the sensor surface allowed the performance of 270 assay cycles within an analysis time of 20 min for each assay cycle. Immunoassays based on a binding inhibition format were performed by using two monoclonal antibodies (MAbs) with different selectivity. Low limits of detection (LODs), in the sub-nanogram per litre range, were attained for DDT-selective (15 ng L−1) and DDT group-selective immunoassays (31 ng L−1). Both assays were carried out in spiked river water samples without significant effect of the matrix. SPR measurements were validated using gas-chromatography–mass spectrometry. The
comparison between methods was in good agreement showing an excellent correlation coefficient (r^2 = 0.995). The SPR analysis of DDT proved
to be three times more sensitive than colorimetric ELISAs without the need of labelling and a much lower time of response. Our SPR biosensor portable platform (β-SPR) is already commercialised by the company SENSIA, S.L. (Spain).|
|Description:||9 pages, 5 figures.-- PMID: 16870422 [PubMed].-- Available online Jul 25, 2006.|
|Publisher version (URL):||http://dx.doi.org/10.1016/j.bios.2006.06.016|
|Appears in Collections:||(IMM-CNM) Artículos|
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