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Title

A specific immunological method to detect and quantify bacterial 2-substituted (1,3)- β-D-glucan

AuthorsWerning, María Laura ; Pérez-Ramos, Adrián; Fernández de Palencia, P. ; Mohedano Bonillo, Mari Luz ; Dueñas, María Teresa; Prieto, Alicia ; López, Paloma
KeywordsExopolysaccharide
Lactic acid bacteria
Beta-D-glucan
Probiotic
Prebiotic
Issue Date26-Nov-2014
PublisherElsevier
CitationCarbohydr Polym. 2014,113:39-45
AbstractExopolysaccharides synthesized by lactic acid bacteria have prebiotic properties and contribute to the rheology and texture of fermented foods. Here, we have standardized an immunological method for the specific detection of 2-substituted (1,3)β-d-glucans. The method allows direct detection and quantification of this exopolysaccharide in culture supernatants containing other mono- and poly-saccharides. Moreover, it allows specific detection of the biomolecules synthesized in vitro in enzymatic reactions. Thus, this method allows the fast identification of producing bacteria, as well as biochemical characterization of the glycosyltransferases responsible for their synthesis.
Description30 p.-1 tab.-5 fig.-2 fig. supl.
Publisher version (URL)http://dx.doi.org/ 10.1016/j.carbpol.2014.06.072
URIhttp://hdl.handle.net/10261/103466
DOI10.1016/j.carbpol.2014.06.072
ISSN0144-8617
E-ISSN1879-1344
Appears in Collections:(CIB) Artículos
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