English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/103295
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Título

Integrated multienzyme electrochemical biosensors for monitoring malolactic fermentation in wines

AutorGamella, M.; Campuzano, Susana ; Conzuelo, F.; Curiel, José Antonio ; Muñoz, Rosario ; Reviejo, A. J.; Pingarrón, José Manuel
Palabras claveEnzyme biosensors
Malolactic fermentation
Self-assembled monolayers
Fecha de publicación15-may-2010
EditorElsevier
CitaciónTalanta 81(3): 925-933 (2010)
ResumenIntegrated amperometric biosensors for the determination of l-malic and l-lactic acids were developed by coimmobilization of the enzymes l-malate dehydrogenase (MDH) and diaphorase (DP), or l-lactate oxidase (LOX) and horseradish peroxidase (HRP), respectively, together with the redox mediator tetrathiafulvalene (TTF), on a 3-mercaptopropionic acid (MPA) self-assembled monolayer (SAM)-modified gold electrode by using a dialysis membrane. The electrochemical oxidation of TTF at +100 mV (vs. Ag/AgCl), and the reduction of TTF+ at -50 mV were used for the monitoring of the enzyme reactions involved in l-malic and l-lactic acid determinations, respectively. Experimental variables concerning the biosensors composition and the detection conditions were optimized for each biosensor. Good relative standard deviation values were obtained in both cases for the measurements carried out with the same biosensor, with no need of cleaning or pretreatment of the bioelectrodes surface, and with different biosensors constructed in the same manner. After 7 days of continuous use, the MDH/DP biosensor still exhibited 90% of the original sensitivity, while the LOX/HRP biosensor yielded a 91% of the original response after 5 days. Calibration graphs for l-malic and l-lactic were obtained with linear ranges of 5.2 × 10-7 to 2.0 × 10-5 and 4.2 × 10-7 to 2.0 × 10-5 M, respectively. The calculated detection limits were 5.2 × 10-7 and 4.2 × 10-7 M, respectively. The biosensors exhibited a high selectivity with no significant interferences. They were applied to monitor malolactic fermentation (MLF) induced by inoculation of Lactobacillus plantarum CECT 748T into a synthetic wine. Samples collected during MLF were assayed for l-malic and l-lactic acids, and the results obtained with the biosensors exhibited a very good correlation when plotted against those obtained by using commercial enzymatic kits. © 2010 Elsevier B.V. All rights reserved.
Versión del editorhttp://dx.doi.org/10.1016/j.talanta.2010.01.038
URIhttp://hdl.handle.net/10261/103295
DOI10.1016/j.talanta.2010.01.038
Identificadoresissn: 0039-9140
Aparece en las colecciones: (IF) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
monitoring_malolactic_Gamella.pdf326,11 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.