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dc.contributor.authorTaboada, Ledicia-
dc.contributor.authorSánchez, Ana Cristina-
dc.contributor.authorVelasco, Amaya-
dc.contributor.authorSantaclara, Francisco J.-
dc.contributor.authorPérez Martín, Ricardo Isaac-
dc.contributor.authorGonzález Sotelo, Carmen-
dc.date.accessioned2014-10-08T11:32:15Z-
dc.date.available2014-10-08T11:32:15Z-
dc.date.issued2014-
dc.identifier.citationJournal of Agricultural and Food Chemistry 62(24): 5699-5706 (2014)es_ES
dc.identifier.issn0021-8561-
dc.identifier.urihttp://hdl.handle.net/10261/103048-
dc.description8 páginas, 5 figuras, 5 tablases_ES
dc.description.abstractSpecies-specific PCR–ELISA assays for the identification of Atlantic cod (Gadus morhua), Alaska pollock (Gadus chalcogrammus), and ling (Molva molva) in food products have been developed. The method, comprising a set of primers common to the first two species, a set of primers for M. molva, and a probe for each species, was designed using ND4 and cytochrome b genes as molecular markers. The sensitivity and selectivity were then determined for each assay. These assays were afterward used to analyze DNA extracted from commercial fish products. The presence of the target species was successfully detected in all analyzed samples, demonstrating the applicability of this method to the analysis of food products.es_ES
dc.description.sponsorshipThe work was supported by the projects “ GENTRASEA: Genetic traceability of fish products. Rapid methods with DNA hybridization probes ” and “ LABELFISH: Atlantic network on genetic control of fi sh and seafood labelling and traceability ” . The Spanish Ministry of Science and Innovation is gratefully acknowledged for the doctoral fellowship to L.Tes_ES
dc.language.isoenges_ES
dc.publisherAmerican Chemical Societyes_ES
dc.rightsopenAccesses_ES
dc.subjectGadus chalcogrammuses_ES
dc.subjectGadus morhuaes_ES
dc.subjectMolva molvaes_ES
dc.subjectSpecies identificationes_ES
dc.subjectPCR−ELISAes_ES
dc.titleIdentification of Atlantic cod (Gadus morhua), ling (Molva molva), and Alaska pollock (Gadus chalcogrammus) by PCR-ELISA using duplex PCRes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1021/jf500173j-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1021/jf500173jes_ES
dc.identifier.e-issn1520-5118-
dc.embargo.terms2015-05-26-
dc.relation.csices_ES
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