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Title

Identification of Atlantic cod (Gadus morhua), ling (Molva molva), and Alaska pollock (Gadus chalcogrammus) by PCR-ELISA using duplex PCR

AuthorsTaboada, Ledicia ; Sánchez, Ana Cristina ; Velasco, Amaya ; Santaclara, Francisco J. ; Pérez Martín, Ricardo Isaac ; González Sotelo, Carmen
KeywordsGadus chalcogrammus
Gadus morhua
Molva molva
Species identification
PCR−ELISA
Issue Date2014
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 62(24): 5699-5706 (2014)
AbstractSpecies-specific PCR–ELISA assays for the identification of Atlantic cod (Gadus morhua), Alaska pollock (Gadus chalcogrammus), and ling (Molva molva) in food products have been developed. The method, comprising a set of primers common to the first two species, a set of primers for M. molva, and a probe for each species, was designed using ND4 and cytochrome b genes as molecular markers. The sensitivity and selectivity were then determined for each assay. These assays were afterward used to analyze DNA extracted from commercial fish products. The presence of the target species was successfully detected in all analyzed samples, demonstrating the applicability of this method to the analysis of food products.
Description8 páginas, 5 figuras, 5 tablas
Publisher version (URL)http://dx.doi.org/10.1021/jf500173j
URIhttp://hdl.handle.net/10261/103048
DOI10.1021/jf500173j
ISSN0021-8561
E-ISSN1520-5118
Appears in Collections:(IIM) Artículos
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