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Título: | Identification of Atlantic cod (Gadus morhua), ling (Molva molva), and Alaska pollock (Gadus chalcogrammus) by PCR-ELISA using duplex PCR |
Autor: | Taboada, Ledicia CSIC; Sánchez, Ana Cristina CSIC ORCID; Velasco, Amaya CSIC; Santaclara, Francisco J. CSIC; Pérez Martín, Ricardo Isaac CSIC ORCID; González Sotelo, Carmen CSIC ORCID | Palabras clave: | Gadus chalcogrammus Gadus morhua Molva molva Species identification PCR−ELISA |
Fecha de publicación: | 2014 | Editor: | American Chemical Society | Citación: | Journal of Agricultural and Food Chemistry 62(24): 5699-5706 (2014) | Resumen: | Species-specific PCR–ELISA assays for the identification of Atlantic cod (Gadus morhua), Alaska pollock (Gadus chalcogrammus), and ling (Molva molva) in food products have been developed. The method, comprising a set of primers common to the first two species, a set of primers for M. molva, and a probe for each species, was designed using ND4 and cytochrome b genes as molecular markers. The sensitivity and selectivity were then determined for each assay. These assays were afterward used to analyze DNA extracted from commercial fish products. The presence of the target species was successfully detected in all analyzed samples, demonstrating the applicability of this method to the analysis of food products. | Descripción: | 8 páginas, 5 figuras, 5 tablas | Versión del editor: | http://dx.doi.org/10.1021/jf500173j | URI: | http://hdl.handle.net/10261/103048 | DOI: | 10.1021/jf500173j | ISSN: | 0021-8561 | E-ISSN: | 1520-5118 |
Aparece en las colecciones: | (IIM) Artículos |
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Identification_Atlantic_cod_Taboada_2014.pdf | 798,5 kB | Adobe PDF | Visualizar/Abrir |
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