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Título: | Immunoreagents and competitive assays to fludioxonil |
Autor: | Abad Fuentes, Antonio CSIC ORCID; Agulló, Consuelo; Esteve Turrillas, Francesc A. CSIC; Abad Somovilla, Antonio; Mercader Badia, Josep Vicent CSIC ORCID | Palabras clave: | Antibodies Rapid method Hapten Active ester Postharvest fungicide |
Fecha de publicación: | 2014 | Editor: | American Chemical Society | Citación: | Journal of agricultural and food chemistry 62: 2742- 2744 (2014) | Resumen: | Fludioxonil is a new-generation fungicide widely used for postharvest fruit protection. The aim of this study was to produce hitherto unreported immunoreagents for Fludioxonil analysis by immunoassay. Derivatives of this agrochemical were synthesized with different linker tethering sites. Those functionalized haptens were activated, and the purified active esters were efficiently conjugated to different carrier proteins for immunogen and assay antigen preparation. Antibodies to Fludioxonil were raised in rabbits, and their selectivity and affinity were characterized, revealing the significance of the linker. Those antibodies were evaluated using homologous and heterologous conjugates by direct and indirect competitive ELISA formats. Finally, a pair of immunoreagents was identified showing an IC50 value for Fludioxonil of 5.7 μg/L. © 2014 . | URI: | http://hdl.handle.net/10261/102975 | DOI: | 10.1021/jf5001716 | Identificadores: | doi: 10.1021/jf5001716 issn: 1520-5118 |
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