Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/10272
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Solution equilibria of the i-motif-forming region upstream of the B-cell lymphoma-2 P1 promoter

AuthorsKhan, Nasiruddin; Aviñó, Anna CSIC ORCID; Tauler, Romà; González, Carlos CSIC ; Eritja Casadellà, Ramón CSIC ORCID ; Gargallo, Raimundo
KeywordsSpectroscopic methods
Thermodynamics
Nucleic acids
Drugs
Chemometrics
Issue DateDec-2007
PublisherElsevier
CitationBiochimie 89(12): 1562-1572 (2007)
AbstractThe 5'-end of the P1 promoter of the B-cell lymphoma-2 (bcl-2) gene contains a highly guaninecytosine-rich region, which has a role in the regulation of bcl-2 transcription. Whereas the guanine-rich region has been the focus of recent studies, little attention has been paid to the cytosine-rich strand. Here we examine the structural transitions of the cytosine-rich sequence by means of acidebase, mole-ratio and melting experiments monitored by molecular absorption, circular dichroism, and NMR spectroscopies. Two intramolecular i-motif structures have been detected in the pH range 2-7, with maximal formation at pH 4 and 6, respectively. At pH 7.6 the majority species has been associated with a hairpin involving Watson-Crick base pairs. Upon addition of the quadruplex-interacting ligand TmPyP4, bcl-2c structures at pH 6.1 and 7.6 yield identical interaction species with stoichiometries 1:2 (DNA:ligand) and logarithms of formation constant 12.4 ± 0.2 and 11.7 ± 0.1, respectively. The initial i-motif structure at pH 6.1 is lost upon interaction with TmPyP4.
Description11 pages, 8 figures.-- PMID: 17850948 [PubMed].-- Printed version published Dec 2007.
Publisher version (URL)http://dx.doi.org/10.1016/j.biochi.2007.07.026
URIhttp://hdl.handle.net/10261/10272
DOI10.1016/j.biochi.2007.07.026
ISSN0300-9084
Appears in Collections:(IQFR) Artículos
(IBMB) Artículos
(IQAC) Artículos




Files in This Item:
File Description SizeFormat
i-motif-forming_region_Khan.pdf1,01 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

SCOPUSTM   
Citations

45
checked on May 20, 2022

WEB OF SCIENCETM
Citations

44
checked on May 17, 2022

Page view(s)

372
checked on May 24, 2022

Download(s)

436
checked on May 24, 2022

Google ScholarTM

Check

Altmetric

Dimensions


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.