Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/10181
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques

AutorHervás, Gonzalo CSIC ORCID ; Frutos, Pilar CSIC ORCID ; Giráldez, Francisco Javier CSIC ORCID ; Mora Martínez, Manuel José; Fernández Díez, Begoña CSIC; Mantecón, Ángel R. CSIC ORCID
Palabras claveIn vitro gas production technique
Rumen inoculum
Storage
Temperatures
Ruminal fermentation
Fecha de publicación2005
EditorElsevier
CitaciónAnimal Feed Science and Technology 123-124(1): 107-118 (2005)
ResumenUse of preserved rumen fluid (RF) as microbial inoculum for in vitro gas production techniques (IVGPT) would be of practical importance when access to ruminally cannulated animals is limited. This experiment was designed to study effects of RF preservation on its subsequent fermentative activity. Four substrates being: alfalfa hay (AH), barley straw NDF (BS-NDF), cellulose and starch were incubated with rumen inoculum obtained from 12 rumen cannulated grazing sheep, of which six (G) received no supplement and the other 6 (G+S) received 500 g/day of a concentrate. Sub-samples of RF were processed without delay (i.e., control) or stored at approximately 0°C in a chest with crushed ice for 3, 6 and 24 h (treatments 3, 6 and 24 h, respectively) or at −18 °C for 24 h in a freezer (treatment 24 F). An IVGPT was used to determine the rate and extent of gas production and DM disappearance. Average fermentation rate (AFR) and extent of degradation in the rumen (ED) were estimated. Incubations with inoculum derived from supplemented sheep (G+S) resulted in faster rates of gas production (with differences, as proportionate increases, of 0.77 for the barley straw NDF; P < 0.01), lower lag times (e.g., 13.2 versus 4.0 h for the starch; P < 0.05), and higher ED values (e.g., 229 versus 331 g/kg for the barley straw NDF or 173 versus 409 g/kg for the cellulose; P < 0.05). Storing RF in ice for 3 or 6 h had no effect on any fermentation parameter. Treatment 24 h did not affect IVGPT parameters when starch and cellulose were fermented, but estimates of rates and ED were lower (P < 0.05) with BS-NDF and AH. Fermentative activity of rumen inoculum was, in general, reduced by freezing (24 F). However, incubations of starch and cellulose were less affected by frozen inoculum than those of AH or BS-NDF. Preservation of RF for up to 6 h in crushed ice may be a practical alternative, when necessary, to use fresh RF as microbial inoculum.
Descripción12 pages, 5 tables.-- Available online Jun 6, 2005.
Versión del editorhttp://dx.doi.org/10.1016/j.anifeedsci.2005.05.004
URIhttp://hdl.handle.net/10261/10181
DOI10.1016/j.anifeedsci.2005.05.004
ISSN0377-8401
Aparece en las colecciones: (IGM) Artículos

Ficheros en este ítem:
Fichero Descripción Tamaño Formato
Embargo. Digital.CSIC..pdf21,67 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

57
checked on 25-mar-2024

WEB OF SCIENCETM
Citations

56
checked on 28-feb-2024

Page view(s)

500
checked on 28-mar-2024

Download(s)

330
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.