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dc.contributor.authorLópez-Torrejón, Gema-
dc.contributor.authorGuerra, Davide-
dc.contributor.authorCatalá, Rafael-
dc.contributor.authorSalinas, Julio-
dc.contributor.authorPozo, Juan Carlos del-
dc.date.accessioned2014-08-26T11:25:35Z-
dc.date.available2014-08-26T11:25:35Z-
dc.date.issued2012-11-15-
dc.identifier.citationJournal of Integrative Plant Biology 2013, 55 (1): 96–107es_ES
dc.identifier.issn1672-9072-
dc.identifier.urihttp://hdl.handle.net/10261/101215-
dc.description33 p.-3 tab.4 fig.es_ES
dc.description.abstractPost-translational modifications (PTMs) chemically and physically alter the properties of proteins, including their folding, subcellular localization, stability, activity, and consequently their function.In spite of their relevance, studies on PTMs in plants are still limited. Small Ubiquitin-like Modifier(SUMO) modification regulates several biological processes by affecting protein-protein interactions,or changing the subcellular localizations of the target proteins. Here, we describe a novel proteomic approach to identify SUMO targets that combines 2-D liquid chromatography, immunodetection, and mass spectrometry (MS) analyses. We have applied this approach to identify nuclear SUMO targets in response to heat shock. Using a bacterial SUMOylation system, we validated that some of the targets identified here are, in fact, labeled with SUMO1. Interestingly, we found that GIGANTEA (GI), a photoperiodic-pathway protein, is modified with SUMO in response to heat shock both in vitro and in vivo.es_ES
dc.description.sponsorshipThis work was supported by the grants S-GEN-0191-2006 (CAM) and BIO2007-62517 (MEC), CSD-2007-00057, and BIO2011-28184-C02-01 to J. C. P., and S-GEN-0191-2006 (CAM), BIO2007-65284 (MEC) and GEN2006-27787-E (MEC) to J. S. G. L. T. was supported by a postdoctoral contract (Comunidad de Madrid).es_ES
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccesses_ES
dc.subjectMass spectrometry;es_ES
dc.subjectPlantses_ES
dc.subjectPost-translational modificationes_ES
dc.subjectProteomicses_ES
dc.subjectSUMOes_ES
dc.titleIdentification of SUMO target by a novel proteomic approach in plantses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1111/jipb.12012-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1111/jipb.12012es_ES
dc.identifier.e-issn1744-7909-
dc.relation.csices_ES
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