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dc.contributor.authorTorné, Josep María-
dc.contributor.authorLoannidis, N. E.-
dc.contributor.authorVeramendi, Jon-
dc.contributor.authorLopera, Oriol-
dc.contributor.authorKotzabasis, K.-
dc.contributor.authorSantos, Mireya-
dc.date.accessioned2014-08-18T10:16:49Z-
dc.date.available2014-08-18T10:16:49Z-
dc.date.issued2011-04-
dc.identifier.citationLight - Harvesting Processes LHP (2011)-
dc.identifier.urihttp://hdl.handle.net/10261/100877-
dc.descriptionTrabajo presentado en elLight - Harvesting Processes LHP, celebrado en Alemania del 10 al 14de abril de 2011.-
dc.description.abstractTransglutaminases (TGases, EC 2.3.2.13) are intra- and extra-cellular enzymes that catalyze post-translational modification of proteins by establishing -(-glutamyl) links and covalent conjugation of polyamines [1]. In chloroplast it is well established that TGases specifically polyaminylate light harvesting antenna proteins of Photosystem II (LHCII, CP29, CP26, CP24) and therefore a role of this enzyme in photosynthesis has been hypothesized. However, the role of TGases in chloroplast is not yet fully understood. Here we report the effect of the over-expression of maize ( Zea mays ) chloroplast TGase (the first cloned plant TGase) [2, 3] in tobacco ( Nicotiana tabacum) chloroplasts [4, 5]. The TGase activity in over-expressers was increased 4 times in comparison to the wild type tobacco plants, which in turn increased the polyamines associated to antenna proteins about 80%. Functional comparison between Wt tobacco and tgz over-expressers in terms of fast fluorescence induction kinetics, non photochemical quenching of the singlet excited state of chlorophyll a, antenna heterogeneity of PSII and other related factors is shown. Both in vivo probing and electron microscopy studies verified thylakoid remodeling for the TGase over-expressers. PSII antenna heterogeneity in vivo changes to a great extent, with an increase of the centers located in grana appressed regions (PSII) at the expense of centers located mainly in stroma thylakoids (PSII). A major increase in the granum size (i.e. a two times increase in the number of stacked layers) with a concomitant decrease of stroma thylakoids is reported.-
dc.rightsclosedAccess-
dc.titleTransglutaminase versus phothosynthesis related processes: Thylakoid remodelling of transplastomic tobacco plants over-expressing a heterologous transglutaminase-
dc.typepóster de congreso-
dc.date.updated2014-08-18T10:16:50Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
Appears in Collections:(CRAG) Comunicaciones congresos
(IDAB) Comunicaciones congresos
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