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A critical point: The problems associated with the variety of criteria to quantify the antioxidant capacity

AuthorsPrieto, Miguel Ángel ; Vázquez, José Antonio ; Murado García, Miguel Anxo
KeywordsAntioxidant capacity
β-carotene method
Mathematical modelling
Kinetic response
Issue Date2014
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 62(24): 5472-5484 (2014)
AbstractThe oxidant action implies interfering in an autocatalytic process, in which no less than five chemical species are present (oxygen, oxidizable substrate, radicals, antioxidants, and oxidation products); furthermore, reactions of first and second order can take place, and interactions can occur, at several levels of the process. The common and incorrect practice is to use the single-time dose–response of an established antioxidant as a calibration curve to compute the equivalent antioxidant capacity of a sample, which is only tested at one single time–dose, assuming too many false aspects as true. Its use is unreasonable, given the availability of computational applications and instrumental equipment that, combined, provide the adequate tools to work with different variables in nonlinear models. The evaluation of the dose–time dependency of the response of the β-carotene method as a case study, using the combination of strong quantification procedures and a high amount of results with lower experimental error (applying microplate readers), reveals the lack of meaning of single-time criteria. Also, it demonstrates that, in most of the reactions, the time-dependent response in the oxidation process is inherently nonlinear and should not be standardized at one single time, because it would lead to unreliable results, hiding the real aspects of the response. In food matrices, the application of single-time criteria causes deficiencies in the control of the antioxidant content. Therefore, it is logical that, in the past decade, researchers have claimed consensus to increase the determination and effectiveness of antioxidant responses
Description13 páginas, 2 tablas, 6 figuras
Publisher version (URL)http://dx.doi.org/10.1021/jf5005995
Appears in Collections:(IIM) Artículos
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