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The tripartite interaction Pseudomonas fluorescens PICF7-olive-Verticillium dahliae: biocontrol performance of an endophytic bacterium and plant responses

AuthorsMercado-Blanco, Jesús ; Schilirò, Elisabetta ; Maldonado-González, María Mercedes ; Prieto, Pilar
Issue DateMar-2012
PublisherEuropean Cooperation in the Field of Scientific and Technical Research
CitationWorkshop Current Aspects of European Endophyte Research (2012)
AbstractResearch efforts in our group have been focused on biological control as one of the measures to be implemented within an integrated management strategy of Verticillium wilt of olive ( Olea europaea L.)(VWO), a disease caused by the soil-borne fungus Verticillium dahliae (1). Pseudomonas spp. strains natural colonizers of olive roots were earlier demonstrated as efficient biological control agents (BCAs) against VWO. Endophytic lifestyle of Pseudomonas fluorescens PICF7 - one of the best BCAs so far identified - was undoubtedly assessed in roots under non-gnotobiotic conditions by combining fluorescently-tagged bacteria, three dimensional root tissue sect ioning and confocal laser scanning microscopy (2). The same strategy enabled us to confirm the simultaneous endophytic colonization of PICF7 and Pseudomonas putida PICP2, another indigenous olive roots BCA, although in vitro-propagated olive plants and a gnotobiotic study-te st system was used in this case. Remarkably, root hairs were demonstrated to play an important role in the endophytic colonization of inner root tissues by both biocontrol bacteria (4). Moreover, PICF7 and PICP2 cells could simultaneously colonize the same root hairs and spots in the intercellular spaces of the root cortex, suggesting a lack of competence between the two strains. Our observations also indicated that surface colonization of intact roots and endophytic establishment by strain PICF7 seem to be required for the effective control of VWO (3). Yet, the mechanism(s) operating in the PICF7-mediated biocontrol of V. dahliae remains mostly unknown. We therefore aim now to elucidate the genetic and molecular processes taking place during the interaction between olive roots and strain PICF7. Suppression subtractive hybridization (SSH) methodology and transcriptome analysis has allowed the identification of genes differentially expressed during the early moments (up to 21 days after bacterization) of the olive root colonization process by PICF7 (5). R esults indicate that PICF7 triggers a range of resistance responses in olive. Moreover, diverse transcription factors implicated in plant signaling pathways for both biotic and abiotic stimulus response have been identified. These studies represent one of the first attempts to unravel gene functions and regulatory networks involved during a woody host-endophytic BCA interaction. Future matters to be investigated for this tripartite interaction will be presented as well.
DescriptionTrabajo presentado en el Workshop Current Aspects of European Endophyte Research (COST Action FA1103: Endophytes in biotechnology and agriculture), celebrado en Reims (Francia) del 28 al 30 de marzo de 2012.
Appears in Collections:(IAS) Comunicaciones congresos
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