Method for obtaining useful information for the diagnosis of neuromuscular diseases

Abstract

Method for obtaining useful information for the diagnosis of neuromuscular diseases. The invention describes a method for obtaining, on the basis of a patient biopsy, new parameters that allow objective diagnosis of different neuromuscular diseases and the degree to which these affect the patient, comprising: -staining the biopsy sample in order to highlight type-I muscle fibres, type-II muscle fibres and endomysium; - obtaining an image of the biopsy sample after staining; - dividing the image into segments to identify the outlines of the muscle fibres; - forming a network in which the muscle fibres constitute nodes and the contacts between muscle fibres constitute the connections between the nodes; - forming a vector characteristic of the biopsy sample, the elements of which are chosen from geometric parameters of the fibres and parameters of the network constructed; - and comparing control and affected biopsy samples by means of ACP using, in each case, the characteristic vector selected.