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Título: | Influence of the physico-chemical characteristics of chito-oligosaccharides (COS) on antioxidant activity |
Autor: | Miralles, Beatriz CSIC ORCID ; Heras, Ángeles | Palabras clave: | MALDI TOF mass spectrometry Antioxidant activity Physico-chemical properties Lysozyme Chitosanase Chitosan |
Fecha de publicación: | 2013 | Editor: | Elsevier | Citación: | Carbohydrate Polymers 97(2): 776-782 (2013) | Resumen: | Chito-oligosaccharides (COS) are being used as important functional materials for many applications due to their bioactivities. The aim of this research has been to assess the relationship between the physicochemical characteristics, average molecular weight (Mw), acetylation degree (DA), polymerization degree (DP) and specially sequence composition determined by MALDI-TOF MS and the antioxidant properties of COS. These oligosaccharides were obtained by enzymatic depolymerization with chitosanase and lysozyme using a specific chitosan and its reacetylated product. The COS fraction below 5 kDa obtained from chitosanase depolymerization showed the highest capacity to scavenge DPPH radicals and to reduce Fe3+. A correlation was found between the relative amount of molecules with a given A/D (acetylated vs deacetylated units) ratio within the COS and their antioxidant activity, which could be used to predict the antioxidant behavior of a fraction of chito-oligosaccharides. © 2013 Elsevier Inc. | URI: | http://hdl.handle.net/10261/100075 | DOI: | 10.1016/j.carbpol.2013.05.035 | Identificadores: | doi: 10.1016/j.carbpol.2013.05.035 issn: 0144-8617 e-issn: 1879-1344 |
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