DSpace Collection:
http://hdl.handle.net/10261/501
2024-03-19T09:13:28ZValidating expression and biological relevance of key transcripts selected from an olive seed RNA-Seq
http://hdl.handle.net/10261/308791
Título: Validating expression and biological relevance of key transcripts selected from an olive seed RNA-Seq
Autor: Castro López, Antonio Jesús; Bullones, Amanda; Lima Cabello, Elena; Fernández-Pozo, Noé; Claros, Gonzalo M.; Alché Ramírez, Juan de Dios
Descripción: Resumen del poster presentado en: XVI Meeting of Plant Molecular Biology - XVI Reunión de Biología Molecular de Plantas. Sevilla 14-16 Septiembre (2022)2023-05-12T07:10:02ZVascular and root tip GPT2 expression mediates the PGI1-independent response of Arabidopsis to small microbial volatiles
http://hdl.handle.net/10261/287606
Título: Vascular and root tip GPT2 expression mediates the PGI1-independent response of Arabidopsis to small microbial volatiles
Autor: Gámez-Arcas, Samuel; Muñoz Pérez, Francisco José; Ricarte-Bermejo, Adriana; Sánchez-López, Ángela María; Baslam, Marouane; Baroja-Fernández, Edurne; Bahaji, Abdellatif; Almagro, Goizeder; Diego, Nuria de; Doležal, Karel; Leal-López- Jesús; León Morcillo, Rafael Jorge; Castillo, Araceli G.; Pozueta Romero, Javier
Resumen: Microorganisms emit a plethora of volatile compounds (VCs) that promote plant growth and
photosynthesis as well as strong developmental and metabolic changes. In Arabidopsis, the
plastidial isoform of phosphoglucose isomerase PGI1 mediates photosynthesis, metabolism and
development, probably due to its involvement in the synthesis of isoprenoid-derived signals in
vascular tissues (Bahaji et al., 2015; Bahaji et al., 2018). Like in wild-type (WT) plants, microbial
VCs promote growth and photosynthesis as well as starch and CK accumulation in PGI1-lacking
pgi1-2 plants (Sánchez-López et al. 2016). A striking alteration in the transcriptome of leaves
of small fungal VC-treated plants involves strong up-regulation of levels of transcripts of GPT2
(At1g61800), a gene that codes for a plastidial G6P/Pi transporter. We hypothesized that the
PGI1-independent response to microbial volatile emissions involves GPT2 action. To test this
hypothesis, we characterized responses of WT, GPT2-null gpt2-1, PGI1-null pgi1-2 and pgi1-
2gpt2-1 plants to small fungal VCs. In addition, we characterized responses of pgi1-2gpt2-1
plants expressing GPT2 under the control of a vascular tissue- and root tip-specific promoter to
small fungal VCs. Results presented in this work provide evidence that, under conditions in which
PGI1 activity is reduced, long-distance action of GPT2 plays an important role in the response
of plants to small VCs through mechanisms involving resetting of the photosynthesis-related
proteome in leaves and complex GPT2 regulation.
Descripción: Resumen del trabajo presentado en el XVI Meeting of Plant Molecular Biology, celebrado en Sevilla (España), del 14 al 16 de septiembre de 20222023-01-26T10:00:02ZPGI1-mediated vascular pentose phosphate pathway activity determines growth, photosynthesis and metabolism through 2-C-methyl-D-erythritol 4-P pathway action in Arabidopsis
http://hdl.handle.net/10261/287598
Título: PGI1-mediated vascular pentose phosphate pathway activity determines growth, photosynthesis and metabolism through 2-C-methyl-D-erythritol 4-P pathway action in Arabidopsis
Autor: Sánchez-López, Ángela María; Bahaji, Abdellatif; Gámez-Arcas, Samuel; Diego, Nuria de; Baroja-Fernández, Edurne; Muñoz Pérez, Francisco José; Almagro, Goizeder; Doležal, Karel; Nováck, Ondřej; Seguí-Simarro, José María; Tabernero-Mendoza, Mercedes; León Morcillo, Rafael Jorge; Pozueta Romero, Javier
Resumen: Phosphoglucose isomerase is involved in the early steps of glycolysis and regeneration of
glucose-6-phosphate pools in the pentose phosphate pathway (PPP). In Arabidopsis, plastidial
phosphoglucose isomerase (PGI1) is an important determinant of growth, metabolism and
photosynthesis, probably due to its involvement in the synthesis of 2-C-methyl-D-erythritol 4-P
(MEP)-derived hormones in root tips and vascular tissues (Bahaji et al., 2015; Bahaji et al., 2018).
To test this hypothesis, we conducted proteomic and metabolic characterization of PGI1-null
pgi1-2 plants. We also characterized pgi1-2 plants ectopically expressing PGI1 under the control
of a root tip- and vascular tissue-specific promoter. Furthermore, we characterized pfk4/pfk5
knockout plants impaired in the early steps of plastidial glycolysis, and pgl3-1 plants with reduced
activity of the plastidial PPP enzyme 6-phosphogluconolactonase 3. The overall data obtained
in this work provide strong evidence that root tip and vascular PGI1-mediated plastidial PPP
determines growth, development and photosynthesis through MEP pathway action.
Descripción: Resumen del trabajo presentado en el XVI Meeting of Plant Molecular Biology, celebrado en Sevilla (España), del 14 al 16 de septiembre de 20222023-01-26T09:30:02ZMycorrhizal inoculation (G. deserticola) enhances plant growth and changes root system morphology in micropropagated Annona cherimola Mill
http://hdl.handle.net/10261/278533
Título: Mycorrhizal inoculation (G. deserticola) enhances plant growth and changes root system morphology in micropropagated Annona cherimola Mill
Autor: Azcón González de Aguilar, Concepción; Padilla, I. G.; Encina, C. L.; Azcón González de Aguilar, Rosario; Barea Navarro, José Miguel
Descripción: Trabajo presentado al Joint Meeting COST 8.21 -8.22: "Novel biotechnological approaches to plant production: from sterile root to mycorrhizosphere, celebrado del 14 al 15 de junio en Pisa, Italia.2022-09-06T07:58:47Z