DSpace Collection:http://hdl.handle.net/10261/447652024-03-29T01:16:57Z2024-03-29T01:16:57ZModification of regulatory systems in Streptomyces to increase antibiotics productionSantamaría, Ramón I.Antoraz, SergioRodríguez, HéctorSánchez de la Nieta, RicardoDíaz, Margaritahttp://hdl.handle.net/10261/2211742020-10-15T01:12:56Z2020-10-14T11:50:44ZTítulo: Modification of regulatory systems in Streptomyces to increase antibiotics production
Autor: Santamaría, Ramón I.; Antoraz, Sergio; Rodríguez, Héctor; Sánchez de la Nieta, Ricardo; Díaz, Margarita
Descripción: Resumen del trabajo presentado al Congreso Nacional de Biotecnología (BIOTEC), celebrado en Vigo del 10 al 13 de junio de 2019.2020-10-14T11:50:44ZGlucanases and ChitinasesRoncero, CesarVázquez de Aldana, Carlos R.http://hdl.handle.net/10261/2210902021-03-15T10:10:46Z2020-10-13T10:53:10ZTítulo: Glucanases and Chitinases
Autor: Roncero, Cesar; Vázquez de Aldana, Carlos R.
Resumen: In many yeast and fungi, β-(1,3)-glucan and chitin are essential components of the cell wall, an important structure that surrounds cells and which is responsible for their mechanical protection and necessary for maintaining the cellular shape. In addition, the cell wall is a dynamic structure that needs to be remodelled along with the different phases of the fungal life cycle or in response to extracellular stimuli. Since β-(1,3)-glucan and chitin perform a central structural role in the assembly of the cell wall, it has been postulated that β-(1,3)-glucanases and chitinases should perform an important function in cell wall softening and remodelling. This review focusses on fungal glucanases and chitinases and their role during fungal morphogenesis.2020-10-13T10:53:10ZCpG Islands: A Historical PerspectiveAntequera, FranciscoBird, Adrianhttp://hdl.handle.net/10261/1833242019-06-06T00:56:56Z2019-06-05T06:23:27ZTítulo: CpG Islands: A Historical Perspective
Autor: Antequera, Francisco; Bird, Adrian
Resumen: The discovery of CpG islands (CGIs) and the study of their structure and properties run parallel to the development of molecular biology in the last two decades of the twentieth century and to the development of high-throughput genomic technologies at the turn of the millennium. First identified as discrete G + C-rich regions of unmethylated DNA in several vertebrates, CGIs were soon found to display additional distinctive chromatin features from the rest of the genome in terms of accessibility and of the epigenetic modifications of their histones. These features, together with their colocalization with promoters and with origins of DNA replication in mammals, highlighted their relevance in the regulation of genomic processes. Recent approaches have shown with unprecedented detail the dynamics and diversity of the epigenetic landscape of CGIs during normal development and under pathological conditions. Also, comparative analyses across species have started revealing how CGIs evolve and contribute to the evolution of the vertebrate genome.2019-06-05T06:23:27ZCdc14 and Chromosome Condensation: Evaluation of the Recruitment of Condensin to Genomic RegionsRamos, FacundoLeonard, JoanneClemente-Blanco, AndrésAragón, Luishttp://hdl.handle.net/10261/1831982019-06-04T00:56:56Z2019-06-03T14:28:26ZTítulo: Cdc14 and Chromosome Condensation: Evaluation of the Recruitment of Condensin to Genomic Regions
Autor: Ramos, Facundo; Leonard, Joanne; Clemente-Blanco, Andrés; Aragón, Luis
Resumen: Chromosome condensation is an essential morphological event required for successful DNA segregation during mitosis. The high level of genome compaction achieved during this process is attained by the evolutionary conserved condensin complex. Recently, several lines of evidences have demonstrated that the mitotic phosphatase Cdc14 is required to ensure condensin loading onto chromosomes. To date several approaches have been used in order to characterize condensin activity and regulation, however these techniques are time-consuming and require complex equipment. In this chapter we described an easy and reliable protocol to analyze Cdc14-dependent condensin loading onto specific genomic DNA regions by using a chromatin immunoprecipitation (ChIP) technique.2019-06-03T14:28:26Z