2024-03-28T23:47:03Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/94472020-03-12T09:59:17Zcom_10261_5063com_10261_5com_10261_36com_10261_4col_10261_5066col_10261_289
Part per trillion determination of atrazine in natural water samples by a surface plasmon resonance immunosensor
Farré, Marinella
Martínez, Elena
Ramón-Azcón, Javier
Navarro Ortega, Alicia
Radjenovic, Jelena
Mauriz, Elba
Lechuga, Laura M.
Marco, María Pilar
Barceló, Damià
Biosensors
SPR inmunosensor
Atrazine
8 pages, 5 figures.-- PMID: 17393154 [PubMed].-- Printed version published in May 2007.
A new immunoassay for continuously monitoring
atrazine in water has been developed. It uses a portable biosensor platform based on surface plasmon resonance (SPR) technology. This immunoassay is based on the binding inhibition format with purified polyclonal antibodies, with
the analyte derivative covalently immobilized on a gold sensor surface. An alkanethiol self-assembled monolayer (SAM) was formed on the gold-coated sensor surface in order to obtain a reusable sensing surface. The low detection
limit for the optimized assay, calculated as the concentration that produces a 10% decrease in the blank signal, is 20 ng/L. A complete assay cycle, including regeneration, is accomplished
in 25 min. Additionally, a study of the matrix effects of different types of wastewater was performed. All measurements were carried out with the SPR sensor system (β-SPR) commercialised by the company Sensia, S.L. (Spain). The small size and low response time of the β-SPR platform would allow it to be used in real contaminated locations. The immunosensor was evaluated and validated by measuring the
atrazine content of 26 natural samples collected from Ebro River. Solid-phase extraction followed by gas chromatography coupled to mass spectrometric detection (SPE–GC–MS) was used to validate the new immunoassay.
2009-01-07T09:36:18Z
2009-01-07T09:36:18Z
2007-03-13
artículo
Analytical and Bioanalytical Chemistry 388(1): 207-214 (2007)
1618-2642
http://hdl.handle.net/10261/9447
10.1007/s00216-007-1214-2
1618-2650
eng
http://dx.doi.org/10.1007/s00216-007-1214-2
closedAccess
Springer