2024-03-29T13:50:31Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/665572016-02-17T13:37:27Zcom_10261_124com_10261_1col_10261_377
Genetic mapping of 1,3-β-glucanase-encoding genes in Saccharomyces cerevisiae
Correa-Bordes, Jaime
Vázquez de Aldana, Carlos R.
San-Segundo, Pedro A.
Rey Iglesias, Francisco del
The map position of three 1,3 β glucanase-encoding genes in S. cerevisiae has been determined following conventional meiotic and mitotic mapping combined with recombinant DNA techniques. EXG1, EXG2 and SSG1 were localized to chromosomes XII, IV and XV, respectively, by hybridizing the cloned genes to Southern blots of chromosomes separated by pulsed-field gel electrophoresis, in conjunction with the rad52-1-dependent chromosome-loss mapping technique. Meiotic tetrad analyses further localized the EXG1 gene 6.1 centimorgans centromere-proximal to CDC25 on the right arm of chromosome XII. EXG2 was positioned between LYS4 and GCN2 on the right arm of chromosome IV, at distances of 6.2 centimorgans from LYS4 and 4.9 centimorgans from GCN2. Finally, the SSG1 locus mapped on the right arm of chromosome XV, about 8.2 centimorgans to the centromere-proximal side of HIS3.
2013-02-13T10:57:48Z
2013-02-13T10:57:48Z
1992
2013-02-13T10:57:48Z
artículo
Current Genetics 22(4): 283-288 (1992)
http://hdl.handle.net/10261/66557
10.1007/BF00317922
eng
closedAccess
Springer