2024-03-28T18:57:34Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2094682021-12-27T15:56:50Zcom_10261_59com_10261_6col_10261_312
Effects of Halophyte Root Exudates and Their Components on Chemotaxis, Biofilm Formation and Colonization of the Halophilic Bacterium Halomonas Anticariensis FP35T
Sampedro, Inmaculada
Pérez-Mendoza, Daniel
Toral, Laura
Palacios, Esther
Arriagada, César
Llamas, Inmaculada
Ministerio de Economía y Competitividad (España)
Chemotaxis
PGPBs
Halophilic bacteria
Salicornia
Biofilms
Root exudates
Oleanolic acid
© 2020 by the authors.
Increase in soil salinity poses an enormous problem for agriculture and highlights the need for sustainable crop production solutions. Plant growth-promoting bacteria can be used to boost the growth of halophytes in saline soils. Salicornia is considered to be a promising salt-accumulating halophyte for capturing large amounts of carbon from the atmosphere. In addition, colonization and chemotaxis could play an important role in Salicornia-microbe interactions. In this study, the role of chemotaxis in the colonization of the halophilic siredophore-producing bacteria, Halomonas anticariensis FP35T, on Salicornia hispanica plants was investigated. The chemotactic response of FP35T to Salicornia root exudates showed optimum dependence at a salt concentration of 5 % NaCl (w/v). Oleanolic acid, the predominant compound in the exudates detected by HPLC and identified by UPLC-HRMS Q-TOF, acts as a chemoattractant. In vitro experiments demonstrated the enhanced positive effects of wild-type H. anticariensis strain FP35T on root length, shoot length, germination and the vigour index of S. hispanica. Furthermore, these positive effects partially depend on an active chemotaxis system, as the chemotaxis mutant H. anticariensis FP35 ΔcheA showed reduced plant growth promotion for all the parameters tested. Overall, our results suggest that chemotaxis responses to root exudates play an important role in interactions between Salicornia and halophilic bacteria, enhance their colonization and boost plant growth promotion. Preliminary results also indicate that root exudates have a positive impact on H. anticariensis FP35T biofilm formation under saline conditions, an effect which totally depends on the presence of the cheA gene.
2020-04-28T14:07:26Z
2020-04-28T14:07:26Z
2020-04-16
2020-04-28T14:07:26Z
artículo
Microorganisms 8(4): 575 (2020)
2076-2607
http://hdl.handle.net/10261/209468
10.3390/microorganisms8040575
2076-2607
http://dx.doi.org/10.13039/501100003329
32316222
Publisher’s version
https://doi.org/10.3390/microorganisms8040575
Sí
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2014-15532
http://creativecommons.org/licenses/by/4.0/
openAccess
Multidisciplinary Digital Publishing Institute