2024-03-29T02:17:47Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/197712009-12-21T23:00:00Zcom_10261_72com_10261_6col_10261_325
Aquaporin functionality in relation to H+-ATPase activity in root cells of Capsicumm annuum, L. grown under salinity.
Martínez-Ballesta, M. Carmen
Martínez, Vicente
Carvajal, Micaela
8 pages, 5 figures, 2 tables.
As water and nutrient uptake should be related in the response of plants to salinity, the aim of this paper is to establish whether or not aquaporin functionality is related to H+-ATPase activity in root cells of pepper (Capsicum annuum L.) plants. Thus, H+-ATPase activity was measured in plasma membrane vesicles isolated from roots and aquaporin functionality was measured using a cell pressure probe in intact roots. Salinity was applied as 60 mM NaCl or 60 mM KCl, to determine which ion (Na+, K+ or Cl−) is producing the effects. We also investigated whether the effects of both salts were ameliorated by Ca2+. Similar results were obtained for cell hydraulic conductivity, Lpc, and H+-ATPase activity, large reductions in the presence at NaCl or KCl and an ameliorative effect of Ca2+. However, fusicoccin (an activator of H+-ATPase) did not alter osmotic water permeability of protoplasts isolated from roots. Addition of Hg2+ inhibited both ATPase and aquaporins, but ATPase also contains Hg-binding sites. Therefore, the results indicate that H+-ATPase and aquaporin activities may not be related in pepper plants.
2009-12-22T08:42:07Z
2009-12-22T08:42:07Z
2003-02-28
artículo
Physiologia Plantarum 117(3): 413-420 (2003)
0885-5684
http://hdl.handle.net/10261/19771
10.1034/j.1399-3054.2003.00044.x
eng
http://dx.doi.org/10.1034/j.1399-3054.2003.00044.x
closedAccess
John Wiley & Sons