2024-03-29T02:11:56Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1908572019-09-27T10:58:01Zcom_10261_94com_10261_8col_10261_473
Specific recombinant gonadotropins induce spermatogenesis and spermiation by first time in a teleost fish, the European EEL
Peñaranda, D. S.
Gallego, V.
Vílchez, M.C.
Rozenfeld, C.
Pérez, Luz
Gómez, Ana
Giménez, Ignacio
Asturiano, Juan F.
Ministerio de Economía y Competitividad (España)
European Commission
Trabajo presentado en el 10º Congreso de la Asociación Ibérica de Endocrinología Comparada (AIEC), celebrado en Castellón (España), del 23 al 25 de septiembre de 2015
Eel recombinant gonadotropins have induced spermatogenesis in vitro, but had
limited effect in vivo. New specific single-chain recombinant gonadotropins
(Rara Avis Biotec S.L.) were tested treating European eel males with rFSH and
rLH. Males received rFSH in three doses (2.8, 1.4 and 0.7 ¿g/fish; high,
medium and low treatments) during 3 weeks. Later, an increasing rLH dose
(every 3 weeks; 1, 2, 6 ¿g/fish) was combined with rFSH. Three
males/treatment were sacrificed every 3 weeks. Blood and testis samples were
taken for steroids and histological analyses and GSI was calculated. Sperm
quality was determined by CASA software.
rFSH by itself was able to induce the spermatogenesis (week 3), causing higher
androgen levels and development until the spermatogonia 2 stage in the high
rFSH group. The rLH (week 6) promoted further maturation to spermatocyte 2
stage in the medium and high groups, but no gonadal progression was observed
in the low group. Higher T levels were reached in all the groups, and the biggest
11KT values in the rFSH high group. A second increase of rLH (week 9)
induced further maturation to spermatid-spermatozoa 1 and spermatocyte 1
stages in the high and low groups, respectively. The medium group showed no
further maturation (spermatocyte 1-2 stages). The highest dose of rLH (week 12)
induced the most advanced stage (spermatozoa 2), and significant GSI increase
in all the groups. A progressive 11KT (high group) and T (all treatments)
decrease was observed from week 9 to 12. All treatments induced spermiating
males, however, the best sperm quality with ?50% motile cells and volumes
~0.4 ml was observed in males treated with the highest rFSH dose and a
progressive increase of rLH treatment. On the other hand, a 20% of nonresponders
were observed and the sperm quality was variable. So, further
experiments are required to improve these treatments.
2019-09-17T08:06:45Z
2019-09-17T08:06:45Z
2015-09-23
comunicación de congreso
10º Congreso de la Asociación Ibérica de Endocrinología Comparada (2015)
http://hdl.handle.net/10261/190857
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000780
eng
Sí
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-41646-R
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