2024-03-28T17:40:07Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1828202021-12-27T16:19:45Zcom_10261_25com_10261_1com_10261_101com_10261_5col_10261_278col_10261_354
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica
Kube, Michael
Chernikova, Tatyana
Al-Ramahi, Yamal
Beloqui, Ana
López-Cortés, Nieves
Guazzaroni, María Eugenia
Heipieper, Hermann J.
Klages, Sven
Kotsyurbenko, Oleg R.
Langer, Inés
Nechitaylo, Taras Y.
Lünsdorf, Heinrich
Fernández, Marisol
Juárez, Silvia
Ciordia, Sergio
Singer, Alexander
Kagan, Olga
Egorova, Olga
Petit, Alain
Stogios, Peter J.
Kim, Youngchang
Tchigvintsev, Anatoli
Flick, Robert
Denaro, Renata
Genovese, María
Albar, Juan Pablo
Reva, Oleg
Martínez-Gomariz, Montserrat
Tran, Hai
Ferrer, Manuel
Savchenko, Alexei
Yakunin, Alexander F.
Yakimov, Michail M.
Golyshina, Olga V.
Reinhardt, Richard
Golyshin, Peter N.
Government of Canada
National Institutes of Health (US)
Max Planck Society
German Research Foundation
European Commission
Functional genomics
Microbial ecology
Comparative genomics
Bacterial genetics
© 2013 Macmillan Publishers Limited
Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis—the paradigm of mesophilic hydrocarbonoclastic bacteria—O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments.
2019-05-30T07:22:58Z
2019-05-30T07:22:58Z
2013-07-23
2019-05-30T07:22:58Z
artículo
Nature Communications 4: 2156 (2013)
2041-1723
http://hdl.handle.net/10261/182820
10.1038/ncomms3156
http://dx.doi.org/10.13039/501100001659
http://dx.doi.org/10.13039/100000002
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100000023
http://dx.doi.org/10.13039/501100004189
23877221
eng
Publisher's version
https://doi.org/10.1038/ncomms3156
info:eu-repo/grantAgreement/EC/FP7/226977
info:eu-repo/grantAgreement/EC/FP7/266473
info:eu-repo/grantAgreement/EC/FP7/245226
info:eu-repo/grantAgreement/EC/FP7/287589
http://creativecommons.org/licenses/by-nc-sa/3.0/
openAccess
Springer Nature