2024-03-28T16:21:54Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1711652020-01-13T12:32:17Zcom_10261_68com_10261_2col_10261_321
First‐Pass Metabolism of Chlorophylls in Mice
Viera Alcaide, Isabel
Chen, Kewei
Ríos, José Julián
Benito, Itziar
Pérez Gálvez, Antonio
Roca, María
Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Absorption
Chlorophylls
Liver
Pheophorbide
SR-BI
33 Páginas; 2 Tablas; 2 Figuras
1 Scope
The dietary intake of chlorophylls is estimated to be ≈50 mg d–1. However, their first pass metabolism and systemic assimilation is not well characterized.
2 Methods and results
A group of 30 mice are fed a diet rich in chlorophylls, while 10 mice received a standard diet without chlorophylls (control group). Liver extracts are analyzed every 15 days by HPLC–ESI(+)/APCI(+)‐hrTOF– MS/MS to measure the accretion of specific chlorophyll metabolites. The chlorophyll profile found in the livers of mice fed a chlorophyll‐rich diet shows that the formation and/or absorption of pheophorbides, pyro‐derivatives, and phytyl‐chlorin e6 require the occurrence of a precise first‐pass metabolism. In addition, the apical absorption of pheorphorbide a‐rich micelles is significantly inhibited in Caucasian colon adenocarcinoma‐2 cells pre‐incubated with BLT1.
3 Conclusion
Pheophorbide a absorption is, at least partly, protein‐mediated through SR‐BI. This active absorption process could explain the specific accumulation of pheophorbide a in the livers of animals fed a chlorophyll‐rich diet. A complementary mechanism could be the de‐esterification of pheophytin a in the liver, yielding pheophorbide a and phytol, which can explain the origin of phytol in the liver. Hence, the results suggest two molecular mechanisms responsible for the accumulation of the health‐promoting compounds pheophorbide and phytol.
2018-10-17T11:25:06Z
2018-10-17T11:25:06Z
2018-09
artículo
Molecular Nutrition and Food Research 62 (17): 1800562 (2018)
1613-4125
http://hdl.handle.net/10261/171165
1613-4133
http://dx.doi.org/10.13039/501100007273
eng
Postprint
http://dx.doi.org/10.1002/mnfr.201800562
Sí
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL 2015‐63890‐R
openAccess
John Wiley & Sons