2024-03-29T00:04:49Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1692012020-12-13T00:07:40Zcom_10261_112com_10261_1com_10261_98com_10261_3col_10261_365col_10261_351
Comprehensive and systematic analysis of the immunocompatibility of polyelectrolyte capsules
Zyuzin, Mikhail V.
Díez, Paula
Goldsmith, Mier
Carregal-Romero, Susana
Teodosio, Cristina
Rejman, Joanna
Feliu, Neus
Escudero, A.
Almendral Parra, María Jesús
Linne, Uwe
Peer, Dan
Fuentes, Manuel
Parak, Wolfgang J.
German Research Foundation
Instituto de Salud Carlos III
Fundación Memoria de D. Samuel Solorzano Barruso
Junta de Castilla y León
Israel National Nanotechnology Initiative
Junta de Andalucía
European Commission
The immunocompability of polyelectrolyte capsules synthesized by layer-by-layer deposition has been investigated. Capsules of different architecture and composed of either non-degradable or biodegradable polymers, with either positively or negatively charged outer surface, and with micrometer size, have been used, and the capsule uptake by different cell lines has been studied and quantified. Immunocompatibility studies were performed with peripheral blood mononuclear cells (PBMCs). Data demonstrate that incubation with capsules, at concentrations relevant for practical applications, did not result in a reduced viability of cells, as it did not show an increased apoptosis. Presence of capsules also did not result in an increased expression of TNF-α, as detected with antibody staining, as well as at mRNA level. It also did not result in increased expression of IL-6, as detected at mRNA level. These results indicate that the polyelectrolyte capsules used in this study are immunocompatible.
2018-08-28T09:41:20Z
2018-08-28T09:41:20Z
2017
2018-08-28T09:42:40Z
artículo
Bioconjugate Chemistry 28(2): 556-564 (2017)
http://hdl.handle.net/10261/169201
10.1021/acs.bioconjchem.6b00657
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100001659
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/501100011011
http://dx.doi.org/10.13039/501100014180
28040889
eng
Sí
info:eu-repo/grantAgreement/EC/FP7/267226
closedAccess
American Chemical Society