2024-03-28T15:37:50Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/158102019-02-26T12:01:13Zcom_10261_5063com_10261_5col_10261_5066
Isolation and identification of neo-clerodane diterpenes from Ajuga remota by high-performance liquid chromatography
Coll Toledano, Josep
Tandrón, Yudelsy A.
HPLC
NMR
Neo-clerodane diterpenes
Anti-feedant activity
Ajuga remota
Labiatae
6 pages, 2 figures, 4 tables.-- PMID: 15688959 [PubMed].-- Printed version published Feb 2005.
In order to explore the suitability of reversed-phase HPLC for the rapid isolation, identification and semipreparative fractionation of neo-clerodane diterpenes from Ajuga remota, an extract from aerial parts of the plant was examined using a C18 column with UV detection and gradient elution with water:methanol. In addition to the known diterpenes ajugarins I, II, IV and V and clerodin, the presence and chromatographic behaviours of ajugapitin, dihydroajugapitin, dihydroclerodin and deacetylajugarin IV, not previously isolated from A. remota, were established. Presence of the epimeric mixture of 14-hydro-15-hydroxyclerodin (scutecyprol A) was also demonstrated together with that of 14-hydro-15-hydroxyajugapitin, the latter only in trace amounts. The structures of all isolated diterpenes were determined from NMR data. The anti-feedant activities of the isolated compounds against Spodoptera littoralis are also reported here.
2009-08-07T07:56:18Z
2009-08-07T07:56:18Z
2005-01-24
artículo
Phytochemical Analysis 16(1): 61-67 (2005)
0958-0344
http://hdl.handle.net/10261/15810
10.1002.pca.812
1099-1565
eng
http://doi.wiley.com/10.1002/pca.812
closedAccess
John Wiley & Sons