2024-03-28T14:50:03Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1484122019-04-26T09:27:36Zcom_10261_127com_10261_1com_10261_105col_10261_380col_10261_358
Growing thrombi release increased levels of CD235a+ microparticles and decreased levels of activated platelet-derived microparticles. Validation in ST-elevation myocardial infarction patients
Suades, R.
Padró Capmany, Teresa
Vilahur, Gemma
Martin-Yuste, V.
Sabaté, M.
Sans-Roselló, J.
Sionis, A.
Badimón Maestro, Lina
Instituto de Salud Carlos III
Ministerio de Sanidad y Consumo (España)
Ministerio de Ciencia e Innovación (España)
European Commission
[Background]
Local fluid dynamics and exposed atherosclerotic lesions regulate thrombus formation. Activated cells in the attached thrombi release microparticles to the circulation (circulating microparticles [cMPs]); however, their phenotype is unknown.
[Objectives]
To investigate the specific phenotype of the cMPs released by growing thrombi.
[Methods/patients]
cMPs released by thrombi growing in different well-characterized thrombogenic conditions were investigated. cMP contents just before and immediately after perfusion of the thrombogenic surfaces were analyzed by triple-labeling flow cytometry. cMPs were tested for their thrombin-generating capacity. The cMPs identified in the ex vivo perfusion experiments were validated in blood of ST-elevation myocardial infarction (STEMI) patients undergoing thrombectomy and percutaneous coronary intervention. Culprit coronary blood (STEMI-CCB) and peripheral artery blood (STEMI-PAB) were simultaneously analyzed and compared with peripheral artery blood from age-matched controls (C-PAB) and peripheral artery blood from patients who had recovered from acute coronary syndrome (ACS) (pSTEMI-PAB).
[Results]
The levels of annexin V+ cMPs significantly increased in blood collected after perfusion of the exposed thrombogenic surfaces. cMP release was directly related to the formed thrombus mass and the plasma procoagulant activity. Post-thrombus blood showed higher thrombin generation potential and contained higher levels of cMPs carrying glycophorin-A (CD235a+; erythrocyte-derived microparticles [ErMPs]) than preperfusion blood (P < 0.05), whereas the levels of cMPs carrying activated and adhesion platelet markers were decreased. STEMI-CCB and STEMI-PAB had significantly higher ErMP levels than control blood (P < 0.005). ErMP levels were also significantly higher in STEMI-PAB than in pSTEMI-PAB, validating the experimental mechanistic studies and suggesting that ErMPs are markers of ongoing coronary thrombosis (C-statistics: 0.950; 95% confidence interval 0.889–1.000; P < 0.001).
[Conclusion]
Glycophorin-A-rich microparticles are released from evolving growing thrombi into the distal perfusing blood, and can be measured in peripheral blood. CD235a+ cMPs may constitute a novel systemic biomarker of ongoing thrombosis.
2017-04-18T09:42:00Z
2017-04-18T09:42:00Z
2015-09-02
2017-04-18T09:42:00Z
artículo
Journal of Thrombosis and Haemostasis 13(10): 1776-1786 (2015)
http://hdl.handle.net/10261/148412
10.1111/jth.13065
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100000780
eng
Postprint
https://doi.org/10.1111/jth.13065
Sí
closedAccess
John Wiley & Sons