2024-03-29T02:16:26Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1428132017-01-21T01:55:47Zcom_10261_11773com_10261_1col_10261_11774
Correction: Characterization of Human Mesenchymal Stem Cells from Ewing Sarcoma Patients. Pathogenetic Implications
Amaral, Ana Teresa
Biscuola, Michele
López-García, María Ángeles
Álava, Enrique de
Ministerio de Ciencia e Innovación (España)
European Commission
Instituto de Salud Carlos III
Fundação para a Ciência e a Tecnologia (Portugal)
Red Temática de Investigación Cooperativa en Cáncer (España)
Associazione Italiana per la Ricerca sul Cancro
Ministero della Salute
Amaral, Ana Teresa et al.
[Background] Ewing Sarcoma (EWS) is a mesenchymal-derived tumor that generally arises in bone and soft tissue. Intensive research regarding the pathogenesis of EWS has been insufficient to pinpoint the early events of Ewing sarcomagenesis. However, the Mesenchymal Stem Cell (MSC) is currently accepted as the most probable cell of origin.
[Materials and Methods] In an initial study regarding a deep characterization of MSC obtained specifically from EWS patients (MSC-P), we compared them with MSC derived from healthy donors (MSC-HD) and EWS cell lines. We evaluated the presence of the EWS-FLI1 gene fusion and EWSR1 gene rearrangements in MSC-P. The presence of the EWS transcript was confirmed by q-RT-PCR. In order to determine early events possibly involved in malignant transformation, we used a multiparameter quantitative strategy that included both MSC immunophenotypic negative/positive markers, and EWS intrinsic phenotypical features. Markers CD105, CD90, CD34 and CD45 were confirmed in EWS samples.
[Results] We determined that MSC-P lack the most prevalent gene fusion, EWSR1-FLI1 as well as EWSR1 gene rearrangements. Our study also revealed that MSC-P are more alike to MSC-HD than to EWS cells. Nonetheless, we also observed that EWS cells had a few overlapping features with MSC. As a relevant example, also MSC showed CD99 expression, hallmark of EWS diagnosis. However, we observed that, in contrast to EWS cells, MSC were not sensitive to the inhibition of CD99.
[Conclusions] In conclusion, our results suggest that MSC from EWS patients behave like MSC-HD and are phenotypically different from EWS cells, thus raising important questions regarding MSC role in sarcomagenesis. © 2014 Amaral et al.
2017-01-20T12:09:31Z
2017-01-20T12:09:31Z
2014-04-03
artículo
PLoS ONE 9(4): e94455 (2014)
1932-6203
http://hdl.handle.net/10261/142813
10.1371/journal.pone.0094455
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/501100001871
http://dx.doi.org/10.13039/501100005010
http://dx.doi.org/10.13039/501100003196
eng
Publisher's version
Amaral, Ana Teresa; Biscuola, Michele; López-García, María Ángeles; Álava, Enrique de. Characterization of human mesenchymal stem cells from Ewing sarcoma patients. Pathogenetic implications. http://doi.org/10.1371/journal.pone.0085814 . http://hdl.handle.net/10261/142805
http://dx.doi.org/10.1371/journal.pone.0094455
Sí
info:eu-repo/grantAgreement/EC/FP7/278742
http://creativecommons.org/licenses/by/4.0/
openAccess
Public Library of Science