2024-03-29T02:05:42Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1232442018-09-04T12:17:29Zcom_10261_112com_10261_1com_10261_25col_10261_1247col_10261_908
Screening of protein-protein and protein-DNA interactions using microarrays: Applications in biomedicine
Casado-Vela, Juan
Fuentes, Manuel
Franco-Zorrilla, José Manuel
Ministerio de Economía y Competitividad (España)
European Commission
Consejo Superior de Investigaciones Científicas (España)
In this report, we focus on two different array-based technologies that enable large-scale screening of protein interactions. First, protein arrays focus on the identification of protein-protein interactions (PPIs). Second, DNA arrays have also evolved to explore the identification of protein-DNA interactions (PDIs), offering novel tools to control key biological processes. Such a tool is termed protein-binding DNA arrays (also protein-DNA arrays or protein-binding microarrays). These two array-based technologies share unrivaled screening capabilities and constitute valid approaches to address biological questions at the molecular level and, eventually, may be used in biomedical applications. Outstanding achievements of these technologies and their eventual application in biomedicine are discussed here, including the identification and characterization of biomarkers, screening of PPIs, detection of protein posttranslational modifications and biofluid profiling. Advantages and limitations of protein arrays, protein-binding arrays, and other proteomic technologies are also discussed here. Finally, we built a list of dedicated databases and on-line resources comprising updated information on human PPIs and PDIs that can serve as a toolbox for researchers in the field. © 2014 Elsevier Inc.
2015-10-09T11:30:06Z
2015-10-09T11:30:06Z
2014
2015-10-09T11:30:09Z
capítulo de libro
Advances in Protein Chemistry and Structural Biology 95(Cap.8): 231-281 (2014)
http://hdl.handle.net/10261/123244
10.1016/B978-0-12-800453-1.00008-7
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003339
eng
Proteomics in Biomedicine and Pharmacology
Sí
closedAccess
Elsevier