2024-03-29T02:00:22Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1221192016-11-23T08:38:36Zcom_10261_75com_10261_6col_10261_328
Desiccated doubled-haploid embryos obtained from microspore culture of barley cv. Igri
Ryan, A. B.
Castillo Alonso, Ana María
Vallés Brau, María Pilar
Sanz Madoz, José Miguel
Cistué Sola, Luis
Ministerio de Educación (Argentina)
Gobierno de Aragón
Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Barley
Desiccation
Embryos
Doubled haploids
Microspores
5 Pags.- 4 Tabls.- 4 Figs.
Barley microspore-derived doubled-haploid embryos have been produced in vitro. The development of embryo desiccation technology will allow long-term storage, germplasm preservation and low delivery cost. Treatment of the microspore-derived embryos was essential to induce desiccation tolerance and to arrest further development and plant regeneration. At the concentrations used, a treatment with trehalose was more efficient than with sucrose, and mannitol was harmful to the embryos. Up to 80% of the desiccated embryos produced complete green plants when transferred to regeneration medium, by the application of a 0.6 m trehalose or a 10–5 m abscisic acid treatment to the embryos in the culture induction medium. The morphology of these plants was similar to plants produced directly from non-desiccated embryos.
2015-09-15T11:07:24Z
2015-09-15T11:07:24Z
1999-08
artículo
Plant Cell Reports 18 (11): 924-928 (1999)
0721-7714
http://hdl.handle.net/10261/122119
10.1007/s002990050685
1432-203X
http://dx.doi.org/10.13039/501100006667
http://dx.doi.org/10.13039/501100007273
http://dx.doi.org/10.13039/501100010067
eng
http://dx.doi.org/10.1007/s002990050685
Sí
closedAccess
Springer