2024-03-29T06:12:43Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1210932020-11-04T14:03:06Zcom_10261_75com_10261_6com_10261_31com_10261_3com_10261_34com_10261_5com_10261_80com_10261_1col_10261_328col_10261_284col_10261_287col_10261_333
In vivo reconstitution of a homodimeric cytochrome b559 like structure: The role of the N-terminus α-subunit from Synechocystis sp. PCC 6803
Luján Serrano, María Ángeles
Martínez, Jesús I.
Alonso, Pablo J.
Torrado, Alejandro
Roncel Gil, Mercedes
Ortega, José M.
Sancho, Javier
Picorel Castaño, Rafael
Ministerio de Economía y Competitividad (España)
Gobierno de Aragón
Junta de Andalucía
European Commission
Alpha-helix
Cytochrome
Membrane
Photosynthesis
Chimeric proteins
Protein assembly
The cytochrome b559 is a heme-bridged heterodimeric protein with two subunits, α and β. Both subunits from Synechocystis sp. PCC 6803 have previously been cloned and overexpressed in Escherichia coli and in vivo reconstitution experiments have been carried out. The formation of homodimers in the bacterial membrane with endogenous heme was only observed in the case of the β-subunit (β/β) but not with the full length α-subunit. In the present work, reconstitution of a homodimer (α/α) cytochrome b559 like structure was possible using a chimeric N-terminus α-subunit truncated before the amino acid isoleucine 17, eliminating completely a short amphipathic α-helix that lays on the surface of the membrane. Overexpression and in vivo reconstitution in the bacteria was clearly demonstrated by the brownish color of the culture pellet and the use of a commercial monoclonal antibody against the fusion protein carrier, the maltoside binding protein, and polyclonal antibodies against a synthetic peptide of the α-subunit from Thermosynechococcus elongatus. Moreover, a simple partial purification after membrane solubilization with Triton X-100 confirmed that the overexpressed protein complex corresponded with the maltoside binding protein-chimeric α-subunit cytochrome b559 like structure. The features of the new structure were determined by UV–Vis, electron paramagnetic resonance and redox potentiometric techniques. Ribbon representations of all possible structures are also shown to better understand the mechanism of the cytochrome b559 maturation in the bacterial cytoplasmic membrane.
2015
artículo
Journal of Photochemistry and Photobiology B: Biology 152: 308-317 (2015)
1011-1344
http://hdl.handle.net/10261/121093
10.1016/j.jphotobiol.2015.07.006
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100010067
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100011011
eng
Postprint
http://dx.doi.org/10.1016/j.jphotobiol.2015.07.006
Sí
http://creativecommons.org/licenses/by-nc-nd/4.0/
openAccess
Elsevier