2024-03-28T22:18:45Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1122032018-10-03T07:52:33Zcom_10261_9676com_10261_8col_10261_9677
Dicer-1 is a key enzyme in the regulation of oogenesis in panoistic ovaries
Donato Tanaca, Erika
Piulachs, Maria-Dolors
Ministerio de Ciencia e Innovación (España)
European Commission
Consejo Superior de Investigaciones Científicas (España)
MicroRNA
Cockroaches
Drosophila
Juvenile hormone
Blattella germanica
[Background information] In insects, the action of microRNAs (miRNAs) on oogenesis has been explored only in dipterans, which possess meroistic ovaries, a highly modified ovarian type. Here we study miRNA function in the most primitive, panoistic type of ovaries using the phylogenetically basal insect Blattella germanica (Dictyoptera, Blattellidae) as model.
[Results] Dicer-1 (Dcr1), a key enzyme in miRNA biogenesis, was depleted using RNAi. Females treated with double-stranded RNA targeting Dicer-1, exhibited deep alterations in oocyte development; among them, the follicular epithelia of the basal oocytes did not develop, thus resulting in sterile females.
[Conclusions] These effects derived from the absence of Dicer-1 suggest that miRNAs are crucial for the regulation of oogenesis in panoistic ovaries, the most primitive insect ovarian type. © 2012 Soçiété Francaise des Microscopies and Société de Biologie Cellulaire de France.
2015-03-11T13:10:33Z
2015-03-11T13:10:33Z
2012-08
2015-03-11T13:10:34Z
artículo
Biology of the Cell 104(8): 452-461 (2012)
http://hdl.handle.net/10261/112203
10.1111/boc.201100044
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003339
eng
http://dx.doi.org/10.1111/boc.201100044
Sí
closedAccess
John Wiley & Sons