2024-03-28T15:14:12Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1047262019-11-19T08:31:56Zcom_10261_80com_10261_1col_10261_333
Starch synthase 4 is located in the thylakoid membrane and interacts with plastoglobule-associated proteins in Arabidopsis
Gámez-Arjona, Francisco M.
Raynaud, Sandy
Ragel, Paula
Mérida, Ángel
Arabidopsis thaliana
Starch synthase
Thylakoids
Plastoglobules
Fibrillins
Protein-protein interactions
Starch synthesis requires the formation of a primer that can be subsequently elongated and branched. How this primer is produced, however, remains unknown. The control of the number of starch granules produced per chloroplast is also a matter of debate. We previously showed starch synthase 4 (SS4) to be involved in both processes, although the mechanisms involved are yet to be fully characterised. The present work shows that SS4 displays a specific localization different from other starch synthases. Thus, this protein is located in specific areas of the thylakoid membrane and interacts with the proteins fibrillin 1a (FBN1a) and 1b (FBN1b), which are mainly located in plastoglobules. SS4 would seem to be associated with plastoglobules attached to the thylakoids (or to that portion of the thylakoids where plastoglobules have originated), forming a complex that includes the FBN1s and other as-yet unidentified proteins. The present results also indicate that the localization pattern of SS4, and its interactions with the FBN1 proteins, are mediated through its N-terminal region, which contains two long coiled-coil motifs. The localization of SS4 in specific areas of the thylakoid membrane suggests that starch granules are originated at specific regions of the chloroplast
2014-11-10T09:45:26Z
2014-11-10T09:45:26Z
2014
artículo
Plant Journal, 80(2);305-316 (2014)
http://hdl.handle.net/10261/104726
10.1111/tpj.12633
eng
Postprint
http://dx.doi.org/10.1111/tpj.12633
Sí
openAccess
John Wiley & Sons