2024-03-28T11:46:32Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/887062016-02-18T02:31:59Zcom_10261_88com_10261_8col_10261_341
Characterization of thirty two microsatellite loci for three Atlanto-Mediterranean echinoderm species
García-Cisneros, Álex
Valero-Jiménez, Claudio
Palacín, Cruz
Pérez-Portela, R.
Sea urchin
Pyrosequencing
Inbreeding
Clonality
Conservation
Starfish
5 páginas, 1 tabla.
Thirty two microsatellites were optimized from
454 pyrosequencing libraries for three Atlanto-Mediterranean
echinoderms: Coscinasterias tenuispina, Echinaster
sepositus and Arbacia lixula. We observed different frequency
of microsatellite types (di-, tri-, tetra- and pentanucleotide)
throughout the genome of the species, but no
significant differences were observed in allele richness
among different microsatellite repeats. No loci showed
linkage disequilibrium. Heterozygosity deficit and departure
from Hardy–Weinberg equilibrium were observed for
some loci, in two species, probably due to high levels of
inbreeding. Heterozygosity excess observed in C. tenuispina
could be explained by selection against homozygotes and/
or outcrossing.
This research was supported by a predoctoral
FPI-MICINN fellowship to A.G.C (BES-2011-044154), the Spanish
Government project CTM2010-22218-C02-02 and the European
project 287844-COCONET (FP7/2007–2013).
Peer reviewed
2013-12-16T13:21:42Z
2013-12-16T13:21:42Z
2013
artículo
http://purl.org/coar/resource_type/c_6501
Conservation Genetics Resources (5) : 749-753 (2013)
1877-7252
http://hdl.handle.net/10261/88706
10.1007/s12686-013-9897-5
1877-7260
en
http://dx.doi.org/10.1007/s12686-013-9897-5
open
Springer