2024-03-29T10:39:42Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/802172022-12-21T11:05:06Zcom_10261_75com_10261_6col_10261_328
Mutually exclusive alterations in secondary metabolism are critical for the uptake of insoluble iron compounds by Arabidopsis and Medicago truncatula
Rodríguez-Celma, Jorge
Lin, WenDar
Fu, GuinMau
Abadía Bayona, Javier
López-Millán, Ana Flor
Schmidt, Wolfgang
The generally low bioavailability of iron in aerobic soil systems forced plants to evolve sophisticated genetic strategies to improve the acquisition of iron from sparingly soluble and immobile iron pools. To distinguish between conserved and species-dependent components of such strategies, we analyzed iron deficiency-induced changes in the transcriptome of two model species, Arabidopsis (Arabidopsis thaliana) and Medicago truncatula. Transcriptional profiling by RNA sequencing revealed a massive upregulation of genes coding for enzymes involved in riboflavin biosynthesis in M. truncatula and phenylpropanoid synthesis in Arabidopsis upon iron deficiency. Coexpression and promoter analysis indicated that the synthesis of flavins and phenylpropanoids is tightly linked to and putatively coregulated with other genes encoding proteins involved in iron uptake. We further provide evidence that the production and secretion of phenolic compounds is critical for the uptake of iron from sources with low bioavailability but dispensable under conditions where iron is readily available. In Arabidopsis, homozygous mutations in the Fe(II)- and 2-oxoglutarate-dependent dioxygenase family gene F69H1 and defects in the expression of PLEIOTROPIC DRUG RESISTANCE9, encoding a putative efflux transporter for products from the phenylpropanoid pathway, compromised iron uptake from an iron source of low bioavailability. Both mutants were partially rescued when grown alongside wild-type Arabidopsis or M. truncatula seedlings, presumably by secreted phenolics and flavins. We concluded that production and secretion of compounds that facilitate the uptake of iron is an essential but poorly understood aspect of the reduction-based iron acquisition strategy, which is likely to contribute substantially to the efficiency of iron uptake in natural conditions.
Peer Reviewed
2013-07-26T10:30:07Z
2013-07-26T10:30:07Z
2013
2013-07-26T10:30:07Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1104/pp.113.220426
issn: 0032-0889
Rodríguez-Celma J, Lin W-D, Fu G-M, Abadía J, López-Millán A-F, Schmidt W. Mutually exclusive alterations in secondary metabolism are critical for the uptake of insoluble iron compounds by Arabidopsis and Medicago truncatula. Plant Physiology 162: 1473-1485 (2013)
http://hdl.handle.net/10261/80217
10.1104/pp.113.220426
23735511
en
http://dx.doi.org/10.1104/pp.113.220426
none
American Society of Plant Biologists