2024-03-29T05:58:36Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/746292021-12-27T15:37:59Zcom_10261_86com_10261_1col_10261_339
Structural analysis of tobacco etch potyvirus HC-pro oligomers involved in aphid transmission
Ruiz-Ferrer, Virginia
Boskovic, Jasminka
Alfonso, Carlos
Rivas, Germán
Llorca, Óscar
López-Abella, Dionisio
López-Moya Gómez, Juan José
Electron microscopy
3D structure
Tobacco etch potyvirus
Oligomeric forms of the HC-Pro protein of the tobacco etch potyvirus (TEV) have been analyzed by analytical ultracentrifugation and single-particle electron microscopy combined with three-dimensional (3D) reconstruction. Highly purified HC-Pro protein was obtained from plants infected with TEV by using a modified version of the virus that incorporates a histidine tag at the HC-Pro N terminus (hisHC-Pro). The purified protein retained a high biological activity in solution when tested for aphid transmission. Sedimentation equilibrium showed that the hisHC-Pro preparations were heterogenous in size. Sedimentation velocity confirmed the previous observation and revealed that the active protein solution contained several sedimenting species compatible with dimers, tetramers, hexamers, and octamers of the protein. Electron microscopy fields of purified protein showed particles of different sizes and shapes. The reconstructed 3D structures suggested that the observed particles could correspond to dimeric, tetrameric, and hexameric forms of the protein. A model of the interactions required for oligomerization of the HC-Pro of potyviruses is proposed. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
This work was supported by Ministerio de ciencia y tecnología (MCyT, Spain) projects AGL2001-2141 to J.J.L.-M. and SAF2002-01715 to O.L., and by Comunidad de Madrid (Spain) grant 07 M-0072-2002 to J.J.L.-M.
Peer Reviewed
2013-04-19T06:48:38Z
2013-04-19T06:48:38Z
2005-03
2013-04-19T06:48:40Z
artículo
http://purl.org/coar/resource_type/c_6501
issn: 0022-538X
Journal of Virology 79(6):3758-3765(2005)
1098-5514
http://hdl.handle.net/10261/74629
10.1128/JVI.79.6.3758-3765.2005
15731269
en
http://dx.doi.org/10.1128/JVI.79.6.3758-3765.2005
none
American Society for Microbiology