2024-03-28T18:45:44Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/571972022-03-25T10:51:14Zcom_10261_47com_10261_8col_10261_300
A sensitive trimethylamine-N-oxide aldolase assay in two steps without deproteinisation
Nielsen, Michael K.
Havemeister, Wiebke
Rehbein, Hartmut
González Sotelo, Carmen
Jørgensen, Bo
TMAOase
Enzyme assay
Fish enzyme
Formaldehyde
4 páginas, 4 figuras
A two-step assay for trimethylamine-N-oxide aldolase (the target enzyme) is described in which the second step, the indicator reaction, is of the enzymatic end-point type. This indicator reaction is carried out at a slightly alkaline pH, outside the target enzyme's active pH range. An effective inactivation of the target enzyme is thus obtained solely by shifting the pH to that of the indicator reaction, thereby avoiding a deproteinisation step. By inserting a downward pH step as the stopping method, many samples may be collected and applied to the indicator reaction simultaneously. These features make the method very productive and easy to perform
This work was supported by grant AIR 3 CT 94-1921
from the European Community.
Peer reviewed
2012-10-03T10:01:48Z
2012-10-03T10:01:48Z
2000
artículo
http://purl.org/coar/resource_type/c_6501
Journal of the Science of Food and Agriculture 80(2): 197-200 (2000)
0022-5142
http://hdl.handle.net/10261/57197
10.1002/(SICI)1097-0010(20000115)80:2<197::AID-JSFA513>3.0.CO;2-U
1097-0010
en
http://dx.doi.org/10.1002/(SICI)1097-0010(20000115)80:2<197::AID-JSFA513>3.0.CO;2-U
none
Wiley-Blackwell