2024-03-28T16:31:45Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/530782019-03-18T15:14:45Zcom_10261_41com_10261_1col_10261_294
H3K27me3 regulates BMP activity in developing spinal cord
Akizu, Naiara
Estarás, Conchi
Guerrero, Laura
Martí, Elisa
Martínez-Balbás, Marian
Ministerio de Educación y Ciencia (España)
BMP pathway
Chick neural tube
Epigenetic regulation
EZH2
Histone methylation
JMJD3
Neural development
During spinal cord development, the combination of secreted signaling proteins and transcription factors provides information for each neural type differentiation. Studies using embryonic stem cells show that trimethylation of lysine 27 of histone H3 (H3K27me3) contributes to repression of many genes key for neural development. However, it remains unclear how H3K27me3-mediated mechanisms control neurogenesis in developing spinal cord. Here, we demonstrate that H3K27me3 controls dorsal interneuron generation by regulation of BMP activity. Our study indicates that expression of Noggin, a BMP extracellular inhibitor, is repressed by H3K27me3. Moreover, we show that Noggin expression is induced by BMP pathway signaling, generating a negative-feedback regulatory loop. In response to BMP pathway activation, JMJD3 histone demethylase interacts with the Smad1/Smad4 complex to demethylate and activate the Noggin promoter. Together, our data reveal how the BMP signaling pathway restricts its own activity in developing spinal cord by modulating H3K27me3 levels at the Noggin promoter. © 2010. Published by The Company of Biologists Ltd.
Este trabajo ha sido financiado por las subvenciones SAF2005-01285, BFU2006-01493, CSD2006-00049 y BFU2007 del Ministerio de Educación y Ciencia del Gobierno Español
Peer Reviewed
2012-07-10T11:02:48Z
2012-07-10T11:02:48Z
2010
2012-07-10T11:02:48Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1242/dev.049395
issn: 0950-1991
e-issn: 1477-9129
Development 137: 2915-2925 (2010)
http://hdl.handle.net/10261/53078
10.1242/dev.049395
en
Postprint
http://dx.doi.org/10.1242/dev.049395
open
Company of Biologists