2024-03-28T22:47:47Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/480172018-08-16T11:45:07Zcom_10261_47com_10261_8col_10261_300
Comparison of diagnostic techniques to detect the clam pathogen Perkinsus olseni
Balseiro, P.
Montes, Jaime
Fernández Conchas, Ramón
Novoa, Beatriz
Figueras Huerta, Antonio
Perkinsus
Diagnosis
Histology
Ray’s fluid thioglycollate medium
Clams
Aquaculture
9 tablas, 4 figuras
Perkinsosis in clams in Galicia (NW Spain) is caused by the protozoan parasite Perkinsus olseni Lester & Davis, 1981. We used 5 clam species of commercial interest cultured in Galicia (Ruditapes decussatus, R. philippinarum, Venerupis pullastra, V. rhomboides, and Donax trunculus) to compare various P. olseni diagnostic techniques. Results of a nested PCR assay for the diagnosis of P. olseni were compared to those obtained using 2 classical methods of diagnosis proposed by the World Organisation for Animal Health (OIE), viz. histology and incubation in Ray’s fluid thioglycollate medium (RFTM). Moreover, the same samples were analyzed by 2 separate research groups. The results obtained by PCR showed high sensitivity and good correlation between research groups. In addition, this method is faster than histopathology and incubation on RFTM and less expensive than histopathology. Moreover, nested PCR requires less specialized training for technicians than histology. Histopathology also showed high specificity and a good correlation between research groups. Results from incubation on RFTM suggest that this method could give divergent results between research groups, particularly in the case of low levels of infection, but it is nevertheless useful for disease-monitoring purposes. PCR is appropriate for rapidly screening large numbers of clams.
This research was supported by Xunta de Galicia (Spain) (PGIDT01MAR40203PR), Secretaría Xeral de Investigación e Desenvolvemento.
Peer reviewed
2012-04-10T07:03:01Z
2012-04-10T07:03:01Z
2010
artículo
http://purl.org/coar/resource_type/c_6501
Diseases of Aquatic Organisms 90(2): 143-151 (2010)
0177-5103
http://hdl.handle.net/10261/48017
10.3354/dao02194
1616-1580
en
http://dx.doi.org/10.3354/dao02194
open
Inter Research