2024-03-29T09:08:09Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/412042020-05-25T07:17:27Zcom_10261_128com_10261_1col_10261_381
Calorie restriction modifies ubiquinone and COQ transcript levels in mouse tissues
Parrado-Fernández, Cristina
López-Lluch, Guillermo
Rodríguez-Bies, Elisabet
Santa-Cruz Calvo, Sara
Navas, Plácido
Ramsey, Jon J.
Villalba, José M.
Junta de Andalucía
Universidad de Córdoba (España)
National Institutes of Health (US)
Calorie restriction
COQ transcripts
Ubiquinone
Ubiquinone biosynthesis
Free radicals
Brain
Myocardium
Coenzyme Q10
9 páginas, 5 figuras, 1 tabla.
We studied ubiquinone (Q), Q homologue ratio, and steady-state levels of mCOQ transcripts in tissues from mice fed ad libitum or under calorie restriction. Maximum ubiquinone levels on a protein basis were found in kidney and heart, followed by liver, brain, and skeletal muscle. Liver and skeletal muscle showed the highest Q9/Q10 ratios with significant interindividual variability. Heart, kidney, and particularly brain exhibited lower Q9/Q10 ratios and interindividual variability. In skeletal muscle and heart, the most abundant mCOQ transcript was mCOQ7, followed by mCOQ8, mCOQ2, mPDSS2, mPDSS1, and mCOQ3. In nonmuscular tissues (liver, kidney, and brain) the most abundant mCOQ transcript was mCOQ2, followed by mCOQ7, mCOQ8, mPDSS1, mPDSS2, and mCOQ3. Calorie restriction increased both ubiquinone homologues and mPDSS2 mRNA in skeletal muscle, but mCOQ7 was decreased. In contrast, Q9 and most mCOQ transcripts were decreased in heart. Calorie restriction also modified the Q9/Q10 ratio, which was increased in kidney and decreased in heart without alterations in mPDSS1 or mPDSS2 transcripts. We demonstrate for the first time that unique patterns of mCOQ transcripts exist in muscular and nonmuscular tissues and that Q and COQ genes are targets of calorie restriction in a tissue-specific way.
This work was supported by NIH Grant 1R01AG028125-01A1 (to J.J.R., P.N., and J.M.V.), Junta de Andalucía Proyectos de Excelencia Grant CVI-00648 (to P.N.), Junta de Andalucía Proyectos de Excelencia Grant P09-CVI-4887 (to J.M.V.), a Junta de Andalucía Proyectos Internacionales grant (to J.M.V.), and BIO-276 (Junta de Andalucía and the University of Córdoba, to J.M.V.). C.P. was funded by Grant CVI-00648.
Peer reviewed
2011-10-17T10:06:55Z
2011-10-17T10:06:55Z
2011-03-27
artículo
http://purl.org/coar/resource_type/c_6501
Free Radical Biology and Medicine 50(12): 1728-1736 (2011)
0891-5849
PMID: 21447381
http://hdl.handle.net/10261/41204
10.1016/j.freeradbiomed.2011.03.024
http://dx.doi.org/10.13039/100000002
http://dx.doi.org/10.13039/501100011011
21447381
en
Postprint
http://dx.doi.org/10.1016/j.freeradbiomed.2011.03.024
Sí
open
Elsevier